Harald Janovjak

2025

A F₄₂₀-dependent Single Domain Chemogenetic Tool for Protein De-dimerization

Antoney, J., Kainrath, S., Dubowsky, J. G., Ahmed, F. H., Kang, S. W., Mackie, E. R. R., Bracho Granado, G., Soares da Costa, T. P., Jackson, C. J. & Janovjak, H., 1 Sept 2025, In: Journal of Molecular Biology. 437, 17, 17 p., 169184.

Research output: Contribution to journal › Article › peer-review

Open Access

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Automated Plate Reader-Based Assays of Light-Activated GPCRs

Gerrard, E. J., Tichy, A.-M. & Janovjak, H., 2025, Optogenetics: Methods and Protocols. Baumschlager, A. (ed.). New York, NY: Springer , p. 217-229 13 p. (Methods in molecular biology (Clifton, N.J.); vol. 2840).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Modular Light-Emitting Diode Shelving Systems for Scalable Optogenetics

Gangemi, C. G. & Janovjak, H., 2025, Optogenetics: Methods and Protocols. Baumschlager, A. (ed.). Springer Nature, Vol. 2840. p. 231-244 14 p. (Methods in Molecular Biology; no. 2840).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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78 Downloads (Pure)

2024

Optogenetics in pancreatic islets: Actuators and effects

Gangemi, C. G. & Janovjak, H., Oct 2024, In: Diabetes. 73, 10, p. 1566-1582 17 p.

Research output: Contribution to journal › Review article › peer-review

Open Access

1 Citation (Scopus)

2023

CDK6 activity in a recurring convergent kinase network motif

Gangemi, C. G., Sabapathy, R. T. & Janovjak, H., Apr 2023, In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 37, 4, 17 p., e22845.

Research output: Contribution to journal › Article › peer-review

Open Access

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170 Downloads (Pure)

Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors

Zhou, F., Tichy, A. M., Imambocus, B. N., Sakharwade, S., Rodriguez Jimenez, F. J., González Martínez, M., Jahan, I., Habib, M., Wilhelmy, N., Burre, V., Lömker, T., Sauter, K., Helfrich-Förster, C., Pielage, J., Grunwald Kadow, I. C., Janovjak, H. & Soba, P., Dec 2023, In: Nature Communications. 14, 1, 8434.

Research output: Contribution to journal › Article › peer-review

Open Access

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4 Citations (Scopus)

17 Downloads (Pure)

2022

Light-activated receptor tyrosine kinases: Designs and applications

Crossman, S. H. & Janovjak, H., Apr 2022, In: Current Opinion in Pharmacology. 63, 7 p., 102197.

Research output: Contribution to journal › Review article › peer-review

8 Citations (Scopus)

Optogenetic neuroregeneration

Janovjak, H. & Kleinlogel, S., Jul 2022, In: Neural Regeneration Research. 17, 7, p. 1468-1470 3 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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3 Citations (Scopus)

60 Downloads (Pure)

Structure-guided optimization of light-activated chimeric G-protein-coupled receptors

Tichy, A. M., So, W. L., Gerrard, E. J. & Janovjak, H., 4 Aug 2022, In: STRUCTURE. 30, 8, p. 1075-1087.e4 17 p.

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

2021

Acute and chronic effects of a light-activated FGF receptor in keratinocytes in vitro and in mice

Rauschendorfer, T., Gurri, S., Heggli, I., Maddaluno, L., Meyer, M., Inglés-Prieto, Á., Janovjak, H. & Werner, S., Nov 2021, In: Life Science Alliance. 4, 11, 12 p., e202101100.

Research output: Contribution to journal › Article › peer-review

Open Access

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7 Citations (Scopus)

65 Downloads (Pure)

A Light-Oxygen-Voltage Receptor Integrates Light and Temperature

Dietler, J., Schubert, R., Krafft, T. G. A., Meiler, S., Kainrath, S., Richter, F., Schweimer, K., Weyand, M., Janovjak, H. & Möglich, A., 23 Jul 2021, In: Journal of Molecular Biology. 433, 15, 20 p., 167107.

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

A Rationally and Computationally Designed Fluorescent Biosensor for d -Serine

Vongsouthi, V., Whitfield, J. H., Unichenko, P., Mitchell, J. A., Breithausen, B., Khersonsky, O., Kremers, L., Janovjak, H., Monai, H., Hirase, H., Fleishman, S. J., Henneberger, C. & Jackson, C. J., 26 Nov 2021, In: ACS Sensors. 6, 11, p. 4193-4205 13 p.

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Formation of Kiss1R/GPER Heterocomplexes Negatively Regulates Kiss1R-mediated Signalling through Limiting Receptor Cell Surface Expression

Ke, R., Lok, S. I. S., Singh, K., Chow, B. K. C., Janovjak, H. & Lee, L. T. O., 2 Apr 2021, In: Journal of Molecular Biology. 433, 7, 16 p., 166843.

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Microbial methionine transporters and biotechnological applications

Mohany, N. A. M., Totti, A., Naylor, K. R. & Janovjak, H., May 2021, In: Applied Microbiology and Biotechnology. 105, 10, p. 3919-3929 11 p.

Research output: Contribution to journal › Review article › peer-review

Open Access

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12 Citations (Scopus)

44 Downloads (Pure)

Optogenetic delivery of trophic signals in a genetic model of Parkinson’s disease

Ingles-Prieto, A., Furthmann, N., Crossman, S. H., Tichy, A. M., Hoyer, N., Petersen, M., Zheden, V., Biebl, J., Reichhart, E., Gyoergy, A., Siekhaus, D. E., Soba, P., Winklhofer, K. F. & Janovjak, H., 15 Apr 2021, In: PloS Genetics. 17, 4 April 2021, 23 p., e1009479.

Research output: Contribution to journal › Article › peer-review

Open Access

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12 Citations (Scopus)

48 Downloads (Pure)

2020

All-Optical Miniaturized Co-culture Assay of Voltage-Gated Ca²⁺ Channels

Agus, V. & Janovjak, H., 2020, Photoswitching Proteins: Methods and Protocols. Niopek, D. (ed.). Humana Press, p. 247-260 14 p. (Methods in Molecular Biology; vol. 2173).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Citation (Scopus)

Design and Application of Light-Regulated Receptor Tyrosine Kinases

Kainrath, S. & Janovjak, H., 2020, Photoswitching Proteins: Methods and Protocols. Niopek, D. (ed.). Humana Press, p. 233-246 14 p. (Methods in Molecular Biology; vol. 2173).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

5 Citations (Scopus)

LTP Induction Boosts Glutamate Spillover by Driving Withdrawal of Perisynaptic Astroglia

Henneberger, C., Bard, L., Panatier, A., Reynolds, J. P., Kopach, O., Medvedev, N. I., Minge, D., Herde, M. K., Anders, S., Kraev, I., Heller, J. P., Rama, S., Zheng, K., Jensen, T. P., Sanchez-Romero, I., Jackson, C. J., Janovjak, H., Ottersen, O. P., Nagelhus, E. A. & Oliet, S. H. R. & 3 others, Stewart, M. G., Nägerl, U. V. & Rusakov, D. A., 9 Dec 2020, In: NEURON. 108, 5, p. 919-936.e11 30 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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161 Citations (Scopus)

97 Downloads (Pure)

Optogenetic control of excitatory post-synaptic differentiation through neuroligin-1 tyrosine phosphorylation

Letellier, M., Lagardère, M., Tessier, B., Janovjak, H. & Thoumine, O., 23 Apr 2020, In: eLife. 9, 21 p., e52027.

Research output: Contribution to journal › Article › peer-review

Open Access

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22 Citations (Scopus)

55 Downloads (Pure)

2019

Editorial overview: Synthetic sensors and signals — new tools for a new trade

Möglich, A. & Janovjak, H., Aug 2019, In: Current Opinion in Structural Biology. 57, p. iii-v 3 p.

Research output: Contribution to journal › Editorial

Engineering Strategy and Vector Library for the Rapid Generation of Modular Light-Controlled Protein–Protein Interactions

Tichy, A. M., Gerrard, E. J., Legrand, J. M. D., Hobbs, R. M. & Janovjak, H., 9 Aug 2019, In: Journal of Molecular Biology. 431, 17, p. 3046-3055 10 p.

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Isolation of synaptic vesicles from genetically engineered cultured neurons

McKenzie, C., Spanova, M., Johnson, A., Kainrath, S., Zheden, V., Sitte, H. H. & Janovjak, H., 15 Jan 2019, In: Journal of Neuroscience Methods. 312, p. 114-121 8 p.

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Light-activated chimeric GPCRs: limitations and opportunities

Tichy, A. M., Gerrard, E. J., Sexton, P. M. & Janovjak, H., Aug 2019, In: Current Opinion in Structural Biology. 57, p. 196-203 8 p.

Research output: Contribution to journal › Review article › peer-review

35 Citations (Scopus)

Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration

Čapek, D., Smutny, M., Tichy, A. M., Morri, M., Janovjak, H. & Heisenberg, C. P., 16 Jan 2019, In: eLife. 8, 24 p., e42093.

Research output: Contribution to journal › Article › peer-review

Open Access

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40 Citations (Scopus)

29 Downloads (Pure)

2018

Monitoring hippocampal glycine with the computationally designed optical sensor GlyFS

Zhang, W. H., Herde, M. K., Mitchell, J. A., Whitfield, J. H., Wulff, A. B., Vongsouthi, V., Sanchez-Romero, I., Gulakova, P. E., Minge, D., Breithausen, B., Schoch, S., Janovjak, H., Jackson, C. J. & Henneberger, C., 1 Sept 2018, In: Nature Chemical Biology. 14, 9, p. 861-869 9 p.

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Optical functionalization of human Class A orphan G-protein-coupled receptors

Morri, M., Sanchez-Romero, I., Tichy, A. M., Kainrath, S., Gerrard, E. J., Hirschfeld, P. P., Schwarz, J. & Janovjak, H., 1 Dec 2018, In: Nature Communications. 9, 1, 1950.

Research output: Contribution to journal › Article › peer-review

Open Access

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50 Citations (Scopus)

48 Downloads (Pure)

2017

Ancestral Protein Reconstruction and Circular Permutation for Improving the Stability and Dynamic Range of FRET Sensors

Clifton, B. E., Whitfield, J. H., Sanchez-Romero, I., Herde, M. K., Henneberger, C., Janovjak, H. & Jackson, C. J., 2017, Synthetic Protein Switches: Methods and Protocols. Stein, V. (ed.). New York: Humana Press, p. 71-87 17 p. (Methods in Molecular Biology; vol. 1596).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

7 Citations (Scopus)

Green-Light-Induced Inactivation of Receptor Signaling Using Cobalamin-Binding Domains

Kainrath, S., Stadler, M., Reichhart, E., Distel, M. & Janovjak, H., 10 Apr 2017, In: Angewandte Chemie - International Edition. 56, 16, p. 4608-4611 4 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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86 Citations (Scopus)

41 Downloads (Pure)

Method for Developing Optical Sensors Using a Synthetic Dye-Fluorescent Protein FRET Pair and Computational Modeling and Assessment

Mitchell, J. A., Zhang, W. H., Herde, M. K., Henneberger, C., Janovjak, H., O’Mara, M. L. & Jackson, C. J., 2017, Synthetic Protein Switches: Methods and Protocols. Stein, V. (ed.). New York: Humana Press, p. 89-99 11 p. (Methods in Molecular Biology; vol. 1596).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Citations (Scopus)

Optogenetic methods in drug screening: technologies and applications

Agus, V. & Janovjak, H., 1 Dec 2017, In: CURRENT OPINION IN BIOTECHNOLOGY. 48, p. 8-14 7 p.

Research output: Contribution to journal › Review article › peer-review

20 Citations (Scopus)

2016

A Phytochrome Sensory Domain Permits Receptor Activation by Red Light

Reichhart, E., Ingles-Prieto, A., Tichy, A. M., McKenzie, C. & Janovjak, H., 17 May 2016, In: Angewandte Chemie - International Edition. 55, 21, p. 6339-6342 4 p.

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Light at the End of the Protein: Crystal Structure of a C-Terminal Light-Sensing Domain

Janovjak, H., 2 Feb 2016, In: STRUCTURE. 24, 2, p. 213-215 3 p.

Research output: Contribution to journal › Short survey › peer-review

Open Access

2 Citations (Scopus)

Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation

Sako, K., Pradhan, S. J., Barone, V., Inglés-Prieto, Á., Müller, P., Ruprecht, V., Čapek, D., Galande, S., Janovjak, H. & Heisenberg, C. P., 19 Jul 2016, In: Cell Reports. 16, 3, p. 866-877 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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91 Citations (Scopus)

48 Downloads (Pure)

Rangefinder: A Semisynthetic FRET Sensor Design Algorithm

Mitchell, J. A., Whitfield, J. H., Zhang, W. H., Henneberger, C., Janovjak, H., O'Mara, M. L. & Jackson, C. J., 23 Nov 2016, In: ACS Sensors. 1, 11, p. 1286-1290 5 p.

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

2015

Construction of a robust and sensitive arginine biosensor through ancestral protein reconstruction

Whitfield, J. H., Zhang, W. H., Herde, M. K., Clifton, B. E., Radziejewski, J., Janovjak, H., Henneberger, C. & Jackson, C. J., Sept 2015, In: Protein Science. 24, 9, p. 1412-1422 11 p.

Research output: Contribution to journal › Article › peer-review

Open Access

58 Citations (Scopus)

Flipping the Photoswitch: Ion Channels Under Light Control

McKenzie, C. K., Sanchez-Romero, I. & Janovjak, H., 2015, Novel Chemical Tools to Study Ion Channel Biology. Ahern, C. & Pless, S. (eds.). Vol. 869. p. 101-117 17 p. (Advances in Experimental Medicine and Biology).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

13 Citations (Scopus)

Light-assisted small-molecule screening against protein kinases

Inglés-Prieto, Á., Reichhart, E., Muellner, M. K., Nowak, M., Nijman, S. M. B., Grusch, M. & Janovjak, H., Dec 2015, In: Nature Chemical Biology. 11, 12, p. 952-954 3 p.

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Quantification of riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in mammalian model cells by CE with LED-induced fluorescence detection

Hühner, J., Ingles-Prieto, Á., Neusüß, C., Lämmerhofer, M. & Janovjak, H., Feb 2015, In: Electrophoresis. 36, 4, p. 518-525 8 p.

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

2014

Spatio-temporally precise activation of engineered receptor tyrosine kinases by light

Grusch, M., Schelch, K., Riedler, R., Reichhart, E., Differ, C., Berger, W., Inglés-Prieto, Á. & Janovjak, H., 1 Aug 2014, In: EMBO Journal. 33, 15, p. 1713-1726 14 p.

Research output: Contribution to journal › Article › peer-review

Open Access

215 Citations (Scopus)

The optogenetic promise for oncology: Episode I

Ingles-Prieto, A., Reichhart, E., Schelch, K., Janovjak, H. & Grusch, M., 2014, In: Molecular and Cellular Oncology. 1, 4, 4 p., e964045.

Research output: Contribution to journal › Article › peer-review

Open Access

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4 Citations (Scopus)

33 Downloads (Pure)

2013

Optical Control of Ligand-Gated Ion Channels

Szobota, S., McKenzie, C. & Janovjak, H., 2013, Ion Channels: Methods and Protocols. Gamper, N. (ed.). p. 417-435 19 p. (Methods in Molecular Biology; vol. 998).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Citations (Scopus)

Optical control of metabotropic glutamate receptors

Levitz, J., Pantoja, C., Gaub, B., Janovjak, H., Reiner, A., Hoagland, A., Schoppik, D., Kane, B., Stawski, P., Schier, A. F., Trauner, D. & Isacoff, E. Y., Apr 2013, In: NATURE NEUROSCIENCE. 16, 4, p. 507-516 10 p.

Research output: Contribution to journal › Article › peer-review

181 Citations (Scopus)

2011

A modern ionotropic glutamate receptor with a K⁺ selectivity signature sequence

Janovjak, H., Sandoz, G. & Isacoff, E. Y., 8 Mar 2011, In: Nature Communications. 2, 1, 5 p., 232.

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Structure-based design of light-controlled proteins

Janovjak, H. & Isacoff, E. Y., 2011, Photosensitive Molecules for Controlling Biological Function. Chambers, J. & Kramer, R. (eds.). p. 233-266 34 p. (Neuromethods; vol. 55).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Citation (Scopus)

2010

A light-gated, potassium-selective glutamate receptor for the optical inhibition of neuronal firing

Janovjak, H., Szobota, S., Wyart, C., Trauner, D. & Isacoff, E. Y., Aug 2010, In: NATURE NEUROSCIENCE. 13, 8, p. 1027-1032 6 p.

Research output: Contribution to journal › Article › peer-review

122 Citations (Scopus)

Pharmacology of ionotropic glutamate receptors: A structural perspective

Stawski, P., Janovjak, H. & Trauner, D., 15 Nov 2010, In: Bioorganic & Medicinal Chemistry. 18, 22, p. 7759-7772 14 p.

Research output: Contribution to journal › Review article › peer-review

56 Citations (Scopus)

2009

Periodic Forces Trigger a Complex Mechanical Response in Ubiquitin

Szymczak, P. & Janovjak, H., 17 Jul 2009, In: Journal of Molecular Biology. 390, 3, p. 443-456 14 p.

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

2008

From valleys to ridges: Exploring the dynamic energy landscape of single membrane proteins

Janovjak, H., Sapra, K. T., Kedrov, A. & Müller, D. J., 2 May 2008, In: Chemphyschem. 9, 7, p. 954-966 13 p.

Research output: Contribution to journal › Review article › peer-review

44 Citations (Scopus)

Fully automated single-molecule force spectroscopy for screening applications

Struckmeier, J., Wahl, R., Leuschner, M., Nunes, J., Janovjak, H., Geisler, U., Hofmann, G., Jähnke, T. & Müller, D. J., 24 Sept 2008, In: Nanotechnology. 19, 38, 384020.

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

2007

Deciphering molecular interactions of native membrane proteins by single-molecule force spectroscopy

Kedrov, A., Janovjak, H., Sapra, K. T. & Müller, D. J., 9 Jun 2007, In: Annual Review of Biophysics and Biomolecular Structure. 36, p. 233-260 28 p.

Research output: Contribution to journal › Review article › peer-review

114 Citations (Scopus)

Professor Harald Janovjak

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