γ-Aminobutyric acid stimulates intrinsic inhibitory and excitatory nerves in the guinea-pig intestine

Anthony Krantis, Marcello Costa, John B. Furness, Joseph Orbach

    Research output: Contribution to journalArticlepeer-review

    114 Citations (Scopus)

    Abstract

    The sites of action of γ-aminobutyric acid (GABA) were examined in preparations of the distal colon and ileum of guinea pigs. GABA caused transient relaxations of the longitudinal and circular muscle of the colon and transient constractions followed by relaxation of the muscle of the ileum. The responses of both parts of the intestine were antagonized by tetrodotoxin and by bicuculline. Nerve-free preparations of the longitudinal muscle of the ileum were not affected by GABA, even in concentrations up to 10−4 g/ml. There was a marked tachyphylaxis of the responses to GABA. Relaxations in response to GABA were not affected by pentolinium or by a combination of phetolamine and propranolol. Contractions in response to GABA were blocked by hyoscine. Neither the ascending excitatory or descending inhibitory components of the peristaltic reflex in the distal colon were affected by bicuculline or by making the intestine tachyphylactic to GABA. The results indicate that GABA stimulates receptor mediating excitation of cholinergic excitatory and enteric inhibitory neurons whereas it has littel or no direct effect on the muscle of the ileum or distal colon.

    Original languageEnglish
    Pages (from-to)461-468
    Number of pages8
    JournalEuropean Journal of Pharmacology
    Volume67
    Issue number4
    DOIs
    Publication statusPublished - 1 Jan 1980

    Keywords

    • Cholinergic nerves
    • Enteric inhibitory nerves
    • GABA
    • Intestinal motility

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