16S rDNA_RFLP分析繁茂膜海绵可培养放线菌的多样性

Translated title of the contribution: 16S rDNA-RFLP analysis of actinomycetes associated with marine sponge Hymeniacidon perleve

Hai-Tao Zhang, Yan Jin, Xing-Ju Yu, Wei Zhang

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

106 culturable symbiotic actinomycetes strains were isolated from intertidal marine sponge Hymeniacidon perleve collected from Chinese Yellow Sea. The 16S rDNA sequences of these strains were amplified and RFLP patterns were analyzed by digestion of 16S rDNA fragments with Hha I. 11 different patterns were identified, with confirmation by the RFLP analysis using Vector NTI simulation. Using Hha I RFLP patterns, the actinomycetes could be identified at the genus level. Phylogentic trees using 16S rDNA sequences were constructed. Majority of actinomycetes belonged to Streptomycetes (78). The other strains belonged to Nocardiopsis (18), Pseudonocardia (3), Nocardia (3), Micromonospora (2), Rhodococcus (1), Actinoalloteichus (1). Strain 177 is the candidate for new species of Actinalloteichus.

Translated title of the contribution16S rDNA-RFLP analysis of actinomycetes associated with marine sponge Hymeniacidon perleve
Original languageChinese (Traditional)
Pages (from-to)828-831
Number of pages4
JournalWei sheng wu xue bao = Acta microbiologica Sinica
Volume45
Issue number6
Publication statusPublished - Dec 2005
Externally publishedYes

Keywords

  • Marine sponge
  • Hymeniacidon perleve
  • Actinomycetes
  • RFLP
  • Diversity

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