106 culturable symbiotic actinomycetes strains were isolated from intertidal marine sponge Hymeniacidon perleve collected from Chinese Yellow Sea. The 16S rDNA sequences of these strains were amplified and RFLP patterns were analyzed by digestion of 16S rDNA fragments with Hha I. 11 different patterns were identified, with confirmation by the RFLP analysis using Vector NTI simulation. Using Hha I RFLP patterns, the actinomycetes could be identified at the genus level. Phylogentic trees using 16S rDNA sequences were constructed. Majority of actinomycetes belonged to Streptomycetes (78). The other strains belonged to Nocardiopsis (18), Pseudonocardia (3), Nocardia (3), Micromonospora (2), Rhodococcus (1), Actinoalloteichus (1). Strain 177 is the candidate for new species of Actinalloteichus.
|Translated title of the contribution||16S rDNA-RFLP analysis of actinomycetes associated with marine sponge Hymeniacidon perleve|
|Original language||Chinese (Traditional)|
|Number of pages||4|
|Journal||Wei sheng wu xue bao = Acta microbiologica Sinica|
|Publication status||Published - Dec 2005|
- Marine sponge
- Hymeniacidon perleve