2-Hexyl-4-Pentylenic Acid (HPTA) Stimulates the Radiotherapy-induced Abscopal Effect on Distal Tumor through Polarization of Tumor-associated Macrophages

Wen Hua DUAN; Li Ya JIN; Zu Chao CAI; David LIM; Zhi Hui FENG

doi:10.3967/bes2021.097

2-Hexyl-4-Pentylenic Acid (HPTA) Stimulates the Radiotherapy-induced Abscopal Effect on Distal Tumor through Polarization of Tumor-associated Macrophages

Wen Hua DUAN, Li Ya JIN, Zu Chao CAI, David LIM, Zhi Hui FENG

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Objective: The aim of this study was to explore the effects of 2-hexyl-4-pentylenic acid (HPTA) in combination with radiotherapy (RT) on distant unirradiated breast tumors. Methods: Using a rat model of chemical carcinogen (7,12-dimethylbenz[a]anthracene,DMBA)-induced breast cancer, tumor volume was monitored and treatment response was evaluated by performing HE staining, immunohistochemistry, immunofluorescence, qRT-PCR, and western blot analyses. Results: The results demonstrated that HPTA in combination with RT significantly delayed the growth of distant, unirradiated breast tumors. The mechanism of action included tumor-associated macrophage (TAM) infiltration into distant tumor tissues, M1 polarization, and inhibition of tumor angiogenesis by IFN-γ. Conclusion: The results suggest that the combination of HPTA with RT has an abscopal effect on distant tumors via M1-polarized TAMs, and HPTA may be considered as a new therapeutic for amplifying the efficacy of local RT for non-targeted breast tumors.

Original language	English
Pages (from-to)	693-704
Number of pages	12
Journal	Biomedical and Environmental Sciences
Volume	34
Issue number	9
DOIs	https://doi.org/10.3967/bes2021.097
Publication status	Published - Sept 2021
Externally published	Yes

Keywords

2-hexyl-4-pentylenic acid (HPTA)
Abscopal effect
Angiogenesis
Breast cancer
Polarization
Radiotherapy (RT)
Tumor-associated macrophages (TAMs)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{df4931a8aead4dcfa22d456f58084503,

title = "2-Hexyl-4-Pentylenic Acid (HPTA) Stimulates the Radiotherapy-induced Abscopal Effect on Distal Tumor through Polarization of Tumor-associated Macrophages",

abstract = "Objective: The aim of this study was to explore the effects of 2-hexyl-4-pentylenic acid (HPTA) in combination with radiotherapy (RT) on distant unirradiated breast tumors. Methods: Using a rat model of chemical carcinogen (7,12-dimethylbenz[a]anthracene,DMBA)-induced breast cancer, tumor volume was monitored and treatment response was evaluated by performing HE staining, immunohistochemistry, immunofluorescence, qRT-PCR, and western blot analyses. Results: The results demonstrated that HPTA in combination with RT significantly delayed the growth of distant, unirradiated breast tumors. The mechanism of action included tumor-associated macrophage (TAM) infiltration into distant tumor tissues, M1 polarization, and inhibition of tumor angiogenesis by IFN-γ. Conclusion: The results suggest that the combination of HPTA with RT has an abscopal effect on distant tumors via M1-polarized TAMs, and HPTA may be considered as a new therapeutic for amplifying the efficacy of local RT for non-targeted breast tumors.",

keywords = "2-hexyl-4-pentylenic acid (HPTA), Abscopal effect, Angiogenesis, Breast cancer, Polarization, Radiotherapy (RT), Tumor-associated macrophages (TAMs)",

author = "DUAN, {Wen Hua} and JIN, {Li Ya} and CAI, {Zu Chao} and David LIM and FENG, {Zhi Hui}",

year = "2021",

month = sep,

doi = "10.3967/bes2021.097",

language = "English",

volume = "34",

pages = "693--704",

journal = "Biomedical and Environmental Sciences",

issn = "0895-3988",

publisher = "Elsevier",

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TY - JOUR

T1 - 2-Hexyl-4-Pentylenic Acid (HPTA) Stimulates the Radiotherapy-induced Abscopal Effect on Distal Tumor through Polarization of Tumor-associated Macrophages

AU - DUAN, Wen Hua

AU - JIN, Li Ya

AU - CAI, Zu Chao

AU - LIM, David

AU - FENG, Zhi Hui

PY - 2021/9

Y1 - 2021/9

N2 - Objective: The aim of this study was to explore the effects of 2-hexyl-4-pentylenic acid (HPTA) in combination with radiotherapy (RT) on distant unirradiated breast tumors. Methods: Using a rat model of chemical carcinogen (7,12-dimethylbenz[a]anthracene,DMBA)-induced breast cancer, tumor volume was monitored and treatment response was evaluated by performing HE staining, immunohistochemistry, immunofluorescence, qRT-PCR, and western blot analyses. Results: The results demonstrated that HPTA in combination with RT significantly delayed the growth of distant, unirradiated breast tumors. The mechanism of action included tumor-associated macrophage (TAM) infiltration into distant tumor tissues, M1 polarization, and inhibition of tumor angiogenesis by IFN-γ. Conclusion: The results suggest that the combination of HPTA with RT has an abscopal effect on distant tumors via M1-polarized TAMs, and HPTA may be considered as a new therapeutic for amplifying the efficacy of local RT for non-targeted breast tumors.

AB - Objective: The aim of this study was to explore the effects of 2-hexyl-4-pentylenic acid (HPTA) in combination with radiotherapy (RT) on distant unirradiated breast tumors. Methods: Using a rat model of chemical carcinogen (7,12-dimethylbenz[a]anthracene,DMBA)-induced breast cancer, tumor volume was monitored and treatment response was evaluated by performing HE staining, immunohistochemistry, immunofluorescence, qRT-PCR, and western blot analyses. Results: The results demonstrated that HPTA in combination with RT significantly delayed the growth of distant, unirradiated breast tumors. The mechanism of action included tumor-associated macrophage (TAM) infiltration into distant tumor tissues, M1 polarization, and inhibition of tumor angiogenesis by IFN-γ. Conclusion: The results suggest that the combination of HPTA with RT has an abscopal effect on distant tumors via M1-polarized TAMs, and HPTA may be considered as a new therapeutic for amplifying the efficacy of local RT for non-targeted breast tumors.

KW - 2-hexyl-4-pentylenic acid (HPTA)

KW - Abscopal effect

KW - Angiogenesis

KW - Breast cancer

KW - Polarization

KW - Radiotherapy (RT)

KW - Tumor-associated macrophages (TAMs)

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U2 - 10.3967/bes2021.097

DO - 10.3967/bes2021.097

M3 - Article

AN - SCOPUS:85116074031

SN - 0895-3988

VL - 34

SP - 693

EP - 704

JO - Biomedical and Environmental Sciences

JF - Biomedical and Environmental Sciences

IS - 9

ER -

2-Hexyl-4-Pentylenic Acid (HPTA) Stimulates the Radiotherapy-induced Abscopal Effect on Distal Tumor through Polarization of Tumor-associated Macrophages

Abstract

Keywords

UN SDGs

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