A cis-carotene derived apocarotenoid regulates etioplast and chloroplast development

Cazzonelli CI, Xin Hou, Yagiz Alagoz, Jhon Rivers, Namraj Dhami, Jiwon Lee, Shashikanth Marri, Barry Pogson

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26 Citations (Scopus)
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Carotenoids are a core plastid component and yet their regulatory function during plastid biogenesis remains enigmatic. A unique carotenoid biosynthesis mutant, carotenoid chloroplast regulation 2 (ccr2), that has no prolamellar body (PLB) and normal PROTOCHLOROPHYLLIDE OXIDOREDUCTASE (POR) levels, was used to demonstrate a regulatory function for carotenoids and their derivatives under varied dark-light regimes. A forward genetics approach revealed how an epistatic interaction between a ζ-carotene isomerase mutant (ziso-155) and ccr2 blocked the biosynthesis of specific cis-carotenes and restored PLB formation in etioplasts. We attributed this to a novel apocarotenoid retrograde signal, as chemical inhibition of carotenoid cleavage dioxygenase activity restored PLB formation in ccr2 etioplasts during skotomorphogenesis. The apocarotenoid acted in parallel to the repressor of photomorphogenesis, DEETIOLATED1 (DET1), to transcriptionally regulate PROTOCHLOROPHYLLIDE OXIDOREDUCTASE (POR), PHYTOCHROME INTERACTING FACTOR3 (PIF3) and ELONGATED HYPOCOTYL5 (HY5). The unknown apocarotenoid signal restored POR protein levels and PLB formation in det1, thereby controlling plastid development.
Original languageEnglish
Article numbere45310.
Number of pages32
Publication statusPublished - 31 Jan 2020
Externally publishedYes

Bibliographical note

Copyright Cazzonelli et al. This article is distributed under the terms of the Creative
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  • cis-carotene
  • apocarotenoid
  • etioplast and chloroplast development
  • genetics


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