A COMPARISON OF SOUTHERN BLOT AND POLYMERASE CHAIN REACTION AS TECHNIQUES TO IDENTIFY LOSS OF DNA HETEROZYGOSITY IN COLORECTAL CANCER

P. J. Hewett, F. Firgaira, A. Morley

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Alterations in the tumour suppressor gene p53 have been found in a wide variety of tumours. The variable number tandem repeat present at the YNZ22 locus is a highly polymorphic marker closely linked to the p53 gene. Examination for loss of heterozygosity at the YNZ22 locus has been carried out by Southern blot or polymerase chain reaction (PCR). A comparison of these techniques shows a 100% concordance rate in normal tissue but a 90.9% rate in tumour tissue. Both false positive and false negative results were obtained. This must be considered in the interpretation of studies using PCR for this purpose.

Original languageEnglish
Pages (from-to)556-557
Number of pages2
JournalAustralian and New Zealand Journal of Surgery
Volume64
Issue number8
DOIs
Publication statusPublished - Aug 1994
Externally publishedYes

Keywords

  • p53
  • polymerase chain reaction
  • Southern blot

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