A COMPARISON OF SOUTHERN BLOT AND POLYMERASE CHAIN REACTION AS TECHNIQUES TO IDENTIFY LOSS OF DNA HETEROZYGOSITY IN COLORECTAL CANCER

P. J. Hewett; F. Firgaira; A. Morley

doi:10.1111/j.1445-2197.1994.tb02285.x

A COMPARISON OF SOUTHERN BLOT AND POLYMERASE CHAIN REACTION AS TECHNIQUES TO IDENTIFY LOSS OF DNA HETEROZYGOSITY IN COLORECTAL CANCER

P. J. Hewett
, F. Firgaira
, A. Morley

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Alterations in the tumour suppressor gene p53 have been found in a wide variety of tumours. The variable number tandem repeat present at the YNZ22 locus is a highly polymorphic marker closely linked to the p53 gene. Examination for loss of heterozygosity at the YNZ22 locus has been carried out by Southern blot or polymerase chain reaction (PCR). A comparison of these techniques shows a 100% concordance rate in normal tissue but a 90.9% rate in tumour tissue. Both false positive and false negative results were obtained. This must be considered in the interpretation of studies using PCR for this purpose.

Original language	English
Pages (from-to)	556-557
Number of pages	2
Journal	Australian and New Zealand Journal of Surgery
Volume	64
Issue number	8
DOIs	https://doi.org/10.1111/j.1445-2197.1994.tb02285.x
Publication status	Published - Aug 1994
Externally published	Yes

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SDG 3 Good Health and Well-being

Keywords

p53
polymerase chain reaction
Southern blot

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abstract = "Alterations in the tumour suppressor gene p53 have been found in a wide variety of tumours. The variable number tandem repeat present at the YNZ22 locus is a highly polymorphic marker closely linked to the p53 gene. Examination for loss of heterozygosity at the YNZ22 locus has been carried out by Southern blot or polymerase chain reaction (PCR). A comparison of these techniques shows a 100\% concordance rate in normal tissue but a 90.9\% rate in tumour tissue. Both false positive and false negative results were obtained. This must be considered in the interpretation of studies using PCR for this purpose.",

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doi = "10.1111/j.1445-2197.1994.tb02285.x",

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AU - Firgaira, F.

AU - Morley, A.

PY - 1994/8

Y1 - 1994/8

N2 - Alterations in the tumour suppressor gene p53 have been found in a wide variety of tumours. The variable number tandem repeat present at the YNZ22 locus is a highly polymorphic marker closely linked to the p53 gene. Examination for loss of heterozygosity at the YNZ22 locus has been carried out by Southern blot or polymerase chain reaction (PCR). A comparison of these techniques shows a 100% concordance rate in normal tissue but a 90.9% rate in tumour tissue. Both false positive and false negative results were obtained. This must be considered in the interpretation of studies using PCR for this purpose.

AB - Alterations in the tumour suppressor gene p53 have been found in a wide variety of tumours. The variable number tandem repeat present at the YNZ22 locus is a highly polymorphic marker closely linked to the p53 gene. Examination for loss of heterozygosity at the YNZ22 locus has been carried out by Southern blot or polymerase chain reaction (PCR). A comparison of these techniques shows a 100% concordance rate in normal tissue but a 90.9% rate in tumour tissue. Both false positive and false negative results were obtained. This must be considered in the interpretation of studies using PCR for this purpose.

KW - p53

KW - polymerase chain reaction

KW - Southern blot

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SN - 0004-8682

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JO - Australian and New Zealand Journal of Surgery

JF - Australian and New Zealand Journal of Surgery

IS - 8

ER -

A COMPARISON OF SOUTHERN BLOT AND POLYMERASE CHAIN REACTION AS TECHNIQUES TO IDENTIFY LOSS OF DNA HETEROZYGOSITY IN COLORECTAL CANCER

Abstract

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Keywords

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