A comparison of the isoelectric points of mouse liver UDP-glucuronosyltransferase enzymes conjugating the twelve benzo[a]pyrene phenols

Ida S. Owens, Peter I. Mackenzie

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10 Citations (Scopus)

Abstract

Solubilized mouse liver microsomes were subjected to chromatofocusing using a pH 9.5 to 6.0 gradient. UDP-glucuronosyltransferase activity was assayed using 12 benzo[a]pyrene phenols as substrates. The rank of microsomal activity for the phenols was as follows: 12 > 10 > 4 > 1 > 7 > 5 > 8 > 9 > 3 > 11 > 6 > 2. Fractions separated on chromatofocusing according to isoelectric point indicated that 3-, 10-, 11-, and 12-hydroxybenzo[a]pyrene were conjugated primarily by a high pI (∼8.5) activity(s), 2-, 6-, 8-, and 9-hydroxybenzo[a]pyrene were conjugated primarily by a low pI (∼6.7) activity(s), and 1-, 4-, 5-, and 7-hydroxybenzo[a]pyrene were conjugated equally well by high and low pI forms.

Original languageEnglish
Pages (from-to)1075-1082
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume109
Issue number4
DOIs
Publication statusPublished - 31 Dec 1982
Externally publishedYes

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