TY - JOUR
T1 - A comparison of the isoelectric points of mouse liver UDP-glucuronosyltransferase enzymes conjugating the twelve benzo[a]pyrene phenols
AU - Owens, Ida S.
AU - Mackenzie, Peter I.
PY - 1982/12/31
Y1 - 1982/12/31
N2 - Solubilized mouse liver microsomes were subjected to chromatofocusing using a pH 9.5 to 6.0 gradient. UDP-glucuronosyltransferase activity was assayed using 12 benzo[a]pyrene phenols as substrates. The rank of microsomal activity for the phenols was as follows: 12 > 10 > 4 > 1 > 7 > 5 > 8 > 9 > 3 > 11 > 6 > 2. Fractions separated on chromatofocusing according to isoelectric point indicated that 3-, 10-, 11-, and 12-hydroxybenzo[a]pyrene were conjugated primarily by a high pI (∼8.5) activity(s), 2-, 6-, 8-, and 9-hydroxybenzo[a]pyrene were conjugated primarily by a low pI (∼6.7) activity(s), and 1-, 4-, 5-, and 7-hydroxybenzo[a]pyrene were conjugated equally well by high and low pI forms.
AB - Solubilized mouse liver microsomes were subjected to chromatofocusing using a pH 9.5 to 6.0 gradient. UDP-glucuronosyltransferase activity was assayed using 12 benzo[a]pyrene phenols as substrates. The rank of microsomal activity for the phenols was as follows: 12 > 10 > 4 > 1 > 7 > 5 > 8 > 9 > 3 > 11 > 6 > 2. Fractions separated on chromatofocusing according to isoelectric point indicated that 3-, 10-, 11-, and 12-hydroxybenzo[a]pyrene were conjugated primarily by a high pI (∼8.5) activity(s), 2-, 6-, 8-, and 9-hydroxybenzo[a]pyrene were conjugated primarily by a low pI (∼6.7) activity(s), and 1-, 4-, 5-, and 7-hydroxybenzo[a]pyrene were conjugated equally well by high and low pI forms.
UR - http://www.scopus.com/inward/record.url?scp=0020306578&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(82)91886-1
DO - 10.1016/0006-291X(82)91886-1
M3 - Article
C2 - 6820278
AN - SCOPUS:0020306578
SN - 0006-291X
VL - 109
SP - 1075
EP - 1082
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -