A DNA Circuit for IsomiR Detection.

Ashley Connolly, Rena Hirani, Amanda Ellis, Matt Trau

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    A synthetic DNA oligonucleotide has been programmed to function as a biological circuit to detect 5′-IsomiRs. The circuit consists of two integrated DNA switches. The first is “activated” when a DNA probe is enzymatically modified by a reverse transcriptase that incorporates nucleotides complementary to the 5′-region of a microRNA (miRNA). Addition of the correct number and sequence of nucleotides enables the probe to assemble into an asymmetric DNA hairpin. The reconfigured hairpin probe then primes an internal polymerisation reaction, resulting in the synthesis of a symmetrical DNA hairpin. This activates the second switch, which then initiates the amplification of reverse-transcribed miRNA through a continuous cycle of DNA nicking and polymerisation. The DNA circuit enables sensitive and rapid detection of femtomoles of a miRNA transcript under isothermal conditions.

    Original languageEnglish
    Pages (from-to)2172-2178
    Number of pages7
    JournalChembiochem
    Volume17
    Issue number22
    DOIs
    Publication statusPublished - 17 Nov 2016

    Keywords

    • DNA
    • isothermal amplification
    • miRNA detection
    • molecular devices
    • nanotechnology

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