A highly efficient strategy for overexpressing circRNAs

Dawei Liu, Vanessa M. Conn, Gregory J. Goodall, Simon J. Conn

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Circular RNAs (circRNAs) constitute an emerging class of widespread, abundant, and evolutionarily conserved noncoding RNA. They play important and diverse roles in cell development, growth, and tumorigenesis, but functions of the majority of circRNAs remain enigmatic. In order to investigate circRNA function it is necessary to manipulate its expression. While various standard approaches exist for circRNA knockdown, here we present cloning vectors for simplifying the laborious process of cloning circRNAs to achieve high-efficiency overexpression in mammalian cell lines.

Original languageEnglish
Title of host publication Circular RNAs
Subtitle of host publicationMethods and Protocols
EditorsChristoph Dieterich, Argyris Papantonis
PublisherHumana Press Inc.
Pages97-105
Number of pages9
ISBN (Electronic)978-1-4939-7562-4
ISBN (Print)978-1-4939-7561-7
DOIs
Publication statusPublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1724
ISSN (Print)1064-3745

Keywords

  • Circular RNA
  • Expression constructs
  • RNA splicing
  • RT-PCR

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  • Cite this

    Liu, D., Conn, V. M., Goodall, G. J., & Conn, S. J. (2018). A highly efficient strategy for overexpressing circRNAs. In C. Dieterich, & A. Papantonis (Eds.), Circular RNAs: Methods and Protocols (pp. 97-105). (Methods in Molecular Biology; Vol. 1724). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7562-4_8