A highly efficient strategy for overexpressing circRNAs

Dawei Liu, Vanessa M. Conn, Gregory J. Goodall, Simon J. Conn

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

16 Citations (Scopus)


Circular RNAs (circRNAs) constitute an emerging class of widespread, abundant, and evolutionarily conserved noncoding RNA. They play important and diverse roles in cell development, growth, and tumorigenesis, but functions of the majority of circRNAs remain enigmatic. In order to investigate circRNA function it is necessary to manipulate its expression. While various standard approaches exist for circRNA knockdown, here we present cloning vectors for simplifying the laborious process of cloning circRNAs to achieve high-efficiency overexpression in mammalian cell lines.

Original languageEnglish
Title of host publication Circular RNAs
Subtitle of host publicationMethods and Protocols
EditorsChristoph Dieterich, Argyris Papantonis
PublisherHumana Press
Number of pages9
ISBN (Electronic)978-1-4939-7562-4
ISBN (Print)978-1-4939-7561-7
Publication statusPublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Circular RNA
  • Expression constructs
  • RNA splicing
  • RT-PCR


Dive into the research topics of 'A highly efficient strategy for overexpressing circRNAs'. Together they form a unique fingerprint.

Cite this