A highly polymorphic STR locus in Cannabis sativa

Hsing Mei Hsieh, Rur Jyun Hou, Li Chin Tsai, Chih Sheng Wei, Su Wen Liu, Li Hung Huang, Yi Chen Kuo, Adrian Linacre, James Chun I. Lee

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We report on the first short tandem repeat (STR) locus to be isolated from the plant Cannabis sativa. The STR locus, isolated by a hybrid-capture enrichment procedure, was found to contain a simple sequence repeat motif of 6bp. This 6bp repeat motif showed no variation in repeat length but with minor variations in repeat unit sequences. The data show the locus to be highly polymorphic with the number of repeat units ranging from 3 to 40 in 108 screened samples. The observed heterozygosity was approximately 87.04%. The forward and reverse primers (CS1F and CS1R) produced no PCR products in cross-reaction study from 20 species of plants, including highly related species such as Humulus japonicus and Nicotiana tabacum. This hexanucleotide repeat DNA locus could be used to identify cannabis samples and predict their genetic relationship as the test is specific to C. sativa and is highly reproducible.

Original languageEnglish
Pages (from-to)53-58
Number of pages6
JournalForensic Science International
Volume131
Issue number1
DOIs
Publication statusPublished - 9 Jan 2003
Externally publishedYes

Keywords

  • Cannabis sativa
  • Microsatellite DNA
  • Short tandem repeat

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  • Cite this

    Hsieh, H. M., Hou, R. J., Tsai, L. C., Wei, C. S., Liu, S. W., Huang, L. H., Kuo, Y. C., Linacre, A., & Lee, J. C. I. (2003). A highly polymorphic STR locus in Cannabis sativa. Forensic Science International, 131(1), 53-58. https://doi.org/10.1016/S0379-0738(02)00395-X