A highly polymorphic STR locus in Cannabis sativa

Hsing Mei Hsieh; Rur Jyun Hou; Li Chin Tsai; Chih Sheng Wei; Su Wen Liu; Li Hung Huang; Yi Chen Kuo; Adrian Linacre; James Chun I. Lee

doi:10.1016/S0379-0738(02)00395-X

A highly polymorphic STR locus in Cannabis sativa

Hsing Mei Hsieh, Rur Jyun Hou, Li Chin Tsai, Chih Sheng Wei, Su Wen Liu, Li Hung Huang, Yi Chen Kuo, Adrian Linacre, James Chun I. Lee

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

We report on the first short tandem repeat (STR) locus to be isolated from the plant Cannabis sativa. The STR locus, isolated by a hybrid-capture enrichment procedure, was found to contain a simple sequence repeat motif of 6bp. This 6bp repeat motif showed no variation in repeat length but with minor variations in repeat unit sequences. The data show the locus to be highly polymorphic with the number of repeat units ranging from 3 to 40 in 108 screened samples. The observed heterozygosity was approximately 87.04%. The forward and reverse primers (CS1F and CS1R) produced no PCR products in cross-reaction study from 20 species of plants, including highly related species such as Humulus japonicus and Nicotiana tabacum. This hexanucleotide repeat DNA locus could be used to identify cannabis samples and predict their genetic relationship as the test is specific to C. sativa and is highly reproducible.

Original language	English
Pages (from-to)	53-58
Number of pages	6
Journal	Forensic Science International
Volume	131
Issue number	1
DOIs	https://doi.org/10.1016/S0379-0738(02)00395-X
Publication status	Published - 9 Jan 2003
Externally published	Yes

Keywords

Cannabis sativa
Microsatellite DNA
Short tandem repeat

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title = "A highly polymorphic STR locus in Cannabis sativa",

abstract = "We report on the first short tandem repeat (STR) locus to be isolated from the plant Cannabis sativa. The STR locus, isolated by a hybrid-capture enrichment procedure, was found to contain a simple sequence repeat motif of 6bp. This 6bp repeat motif showed no variation in repeat length but with minor variations in repeat unit sequences. The data show the locus to be highly polymorphic with the number of repeat units ranging from 3 to 40 in 108 screened samples. The observed heterozygosity was approximately 87.04%. The forward and reverse primers (CS1F and CS1R) produced no PCR products in cross-reaction study from 20 species of plants, including highly related species such as Humulus japonicus and Nicotiana tabacum. This hexanucleotide repeat DNA locus could be used to identify cannabis samples and predict their genetic relationship as the test is specific to C. sativa and is highly reproducible.",

keywords = "Cannabis sativa, Microsatellite DNA, Short tandem repeat",

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T1 - A highly polymorphic STR locus in Cannabis sativa

AU - Hsieh, Hsing Mei

AU - Hou, Rur Jyun

AU - Tsai, Li Chin

AU - Wei, Chih Sheng

AU - Liu, Su Wen

AU - Huang, Li Hung

AU - Kuo, Yi Chen

AU - Linacre, Adrian

AU - Lee, James Chun I.

PY - 2003/1/9

Y1 - 2003/1/9

N2 - We report on the first short tandem repeat (STR) locus to be isolated from the plant Cannabis sativa. The STR locus, isolated by a hybrid-capture enrichment procedure, was found to contain a simple sequence repeat motif of 6bp. This 6bp repeat motif showed no variation in repeat length but with minor variations in repeat unit sequences. The data show the locus to be highly polymorphic with the number of repeat units ranging from 3 to 40 in 108 screened samples. The observed heterozygosity was approximately 87.04%. The forward and reverse primers (CS1F and CS1R) produced no PCR products in cross-reaction study from 20 species of plants, including highly related species such as Humulus japonicus and Nicotiana tabacum. This hexanucleotide repeat DNA locus could be used to identify cannabis samples and predict their genetic relationship as the test is specific to C. sativa and is highly reproducible.

AB - We report on the first short tandem repeat (STR) locus to be isolated from the plant Cannabis sativa. The STR locus, isolated by a hybrid-capture enrichment procedure, was found to contain a simple sequence repeat motif of 6bp. This 6bp repeat motif showed no variation in repeat length but with minor variations in repeat unit sequences. The data show the locus to be highly polymorphic with the number of repeat units ranging from 3 to 40 in 108 screened samples. The observed heterozygosity was approximately 87.04%. The forward and reverse primers (CS1F and CS1R) produced no PCR products in cross-reaction study from 20 species of plants, including highly related species such as Humulus japonicus and Nicotiana tabacum. This hexanucleotide repeat DNA locus could be used to identify cannabis samples and predict their genetic relationship as the test is specific to C. sativa and is highly reproducible.

KW - Cannabis sativa

KW - Microsatellite DNA

KW - Short tandem repeat

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VL - 131

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EP - 58

JO - Forensic Science International

JF - Forensic Science International

SN - 0379-0738

IS - 1

ER -

A highly polymorphic STR locus in Cannabis sativa

Abstract

Keywords

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