A method for non-invasive genotyping of APCmin/+ mice using fecal samples

Erin Symonds, Michael Fenech

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    The APC min/+ mouse is commonly used in cancer research and is just one of many genetically altered models that is currently being developed. With high numbers of breeding programs, it is important to have a simple method that can be used to genotype the mice non-invasively. Here we report a reproducible method for genotyping mice with DNA extracted from fecal samples. Comparison of fecal results with those obtained from intestinal tissue DNA and clinical outcome (presence/absence of tumors) showed this technique to have 100% accuracy. This non-invasive method of genotyping may be applied to other transgenic mouse models.

    Original languageEnglish
    Article number1
    Pages (from-to)1
    Number of pages3
    JournalBIOLOGICAL PROCEDURES ONLINE
    Volume14
    Issue number1
    DOIs
    Publication statusPublished - 2012

    Keywords

    • APC
    • cancer
    • feces
    • genotyping
    • non-invasive

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