A method for non-invasive genotyping of APCmin/+ mice using fecal samples

Erin Symonds; Michael Fenech

doi:10.1186/1480-9222-14-1

A method for non-invasive genotyping of APCmin/+ mice using fecal samples

Erin Symonds, Michael Fenech

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The APC ^min/+ mouse is commonly used in cancer research and is just one of many genetically altered models that is currently being developed. With high numbers of breeding programs, it is important to have a simple method that can be used to genotype the mice non-invasively. Here we report a reproducible method for genotyping mice with DNA extracted from fecal samples. Comparison of fecal results with those obtained from intestinal tissue DNA and clinical outcome (presence/absence of tumors) showed this technique to have 100% accuracy. This non-invasive method of genotyping may be applied to other transgenic mouse models.

Original language	English
Article number	1
Pages (from-to)	1
Number of pages	3
Journal	BIOLOGICAL PROCEDURES ONLINE
Volume	14
Issue number	1
DOIs	https://doi.org/10.1186/1480-9222-14-1
Publication status	Published - 2012

Keywords

APC
cancer
feces
genotyping
non-invasive

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10.1186/1480-9222-14-1

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KW - non-invasive

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A method for non-invasive genotyping of APCmin/+ mice using fecal samples

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Keywords

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