A method for visualization and isolation of elastic fibres in annulus fibrosus of the disc

Javad Tavakoli; John Costi

doi:10.1016/j.msec.2018.08.007

A method for visualization and isolation of elastic fibres in annulus fibrosus of the disc

Javad Tavakoli, John Costi

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A simple and cost effective protocol for visualization and isolation of the elastic fibres network in the annulus fibrosus (AF) of the disc is explained, to provide other researchers a method that can be applied in disc ultra-structural analysis, biomechanical assessment of elastic fibre and tissue engineered scaffold fabrication. This protocol is developed based on simultaneous sonication and alkali digestion of tissue that eliminates all matrix constituents except for elastic fibres, which is applicable for different species including human. Thin samples harvested from ovine, bovine, porcine and human, which are commonly used in disc research, were exposed to 0.5 M sodium hydroxide solution along with sonication (25 kHz) in distilled water for defined periods of time at room temperature. Post heat treatment removed collagen fibres via the gelatinization process, for visualization of elastic fibres.

Original language	English
Pages (from-to)	299-304
Number of pages	6
Journal	MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
Volume	93
Early online date	4 Aug 2018
DOIs	https://doi.org/10.1016/j.msec.2018.08.007
Publication status	Published - 1 Dec 2018

Keywords

Elastic fibres
Intervertebral disc
Nanostructure
Scanning electron microscopy
Tissue engineering

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abstract = "A simple and cost effective protocol for visualization and isolation of the elastic fibres network in the annulus fibrosus (AF) of the disc is explained, to provide other researchers a method that can be applied in disc ultra-structural analysis, biomechanical assessment of elastic fibre and tissue engineered scaffold fabrication. This protocol is developed based on simultaneous sonication and alkali digestion of tissue that eliminates all matrix constituents except for elastic fibres, which is applicable for different species including human. Thin samples harvested from ovine, bovine, porcine and human, which are commonly used in disc research, were exposed to 0.5 M sodium hydroxide solution along with sonication (25 kHz) in distilled water for defined periods of time at room temperature. Post heat treatment removed collagen fibres via the gelatinization process, for visualization of elastic fibres.",

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AU - Costi, John

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A method for visualization and isolation of elastic fibres in annulus fibrosus of the disc

Abstract

Keywords

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