A model of glaucoma induced by circumlimbal suture in rats and mice

Zheng He, Da Zhao, Anna K. van Koeverden, Christine T. Nguyen, Jeremiah K.H. Lim, Vickie H.Y. Wong, Algis J. Vingrys, Bang V. Bui

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Abstract

The circumlimbal suture is a technique for inducing experimental glaucoma in rodents by chronically elevating intraocular pressure (IOP), a well-known risk factor for glaucoma. This protocol demonstrates a step-by-step guide on this technique in Long Evans rats and C57BL/6 mice. Under general anesthesia, a "purse-string" suture is applied on the conjunctiva, around the equator and behind the limbus of the eye. The fellow eye serves as an untreated control. Over the duration of our study, which was a period of 8 weeks for rats and 12 weeks for mice, IOP remained elevated, as measured regularly by rebound tonometry in conscious animals without topical anesthesia. In both species, the sutured eyes showed electroretinogram features consistent with preferential inner retinal dysfunction. Optical coherence tomography showed selective thinning of the retinal nerve fiber layer. Histology of the rat retina in cross-section found reduced cell density in the ganglion cell layer, but no change in other cellular layers. Staining of flat-mounted mouse retinae with a ganglion cell specific marker (RBPMS) confirmed ganglion cell loss. The circumlimbal suture is a simple, minimally invasive and cost-effective way to induce ocular hypertension that leads to ganglion cell injury in both rats and mice.

Original languageEnglish
Article numbere58287
JournalJournal of Visualized Experiments
Volume2018
Issue number140
Early online date5 Oct 2018
DOIs
Publication statusPublished - 5 Oct 2020
Externally publishedYes

Keywords

  • Animal model
  • Chronic ocular hypertension
  • Circumlimbal suture
  • Glaucoma
  • Intraocular pressure
  • Issue 140
  • Medicine
  • Retinal ganglion cells

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  • Cite this

    He, Z., Zhao, D., van Koeverden, A. K., Nguyen, C. T., Lim, J. K. H., Wong, V. H. Y., Vingrys, A. J., & Bui, B. V. (2020). A model of glaucoma induced by circumlimbal suture in rats and mice. Journal of Visualized Experiments, 2018(140), [e58287]. https://doi.org/10.3791/58287