A NAC domain protein interacts with Tomato leaf curl virus replication accessory protein and enhances viral replication

Luke A. Selth, Satish C. Dogra, M. Saif Rasheed, Helen Healy, John W. Randles, M. Ali Rezaian

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Geminivirus replication enhancer (REn) proteins dramatically increase the accumulation of viral DNA species by an unknown mechanism. In this study, we present evidence implicating SINAC1, a new member of the NAC domain protein family from tomato (Solanum lycopersicum), in Tomato leaf curl virus (TLCV) REn function. We isolated SINAC1 using yeast (Saccharomyces cerevisiae) two-hybrid technology and TLCV REn as bait, and confirmed the interaction between these proteins in vitro. TLCV induces SINAC1 expression specifically in infected cells, and this upregulation requires REn. In a transient TLCV replication system, overexpression of SINAC1 resulted in a substantial increase in viral DNA accumulation. SINAC1 colocalized with REn to the nucleus and activated transcription of a reporter gene in yeast, suggesting that in healthy cells it functions as a transcription factor. Together, these results imply that SINAC1 plays an important role in the process by which REn enhances TLCV replication.

Original languageEnglish
Pages (from-to)311-325
Number of pages15
JournalPlant Cell
Volume17
Issue number1
DOIs
Publication statusPublished - 1 Jan 2005
Externally publishedYes

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