A NAC domain protein interacts with Tomato leaf curl virus replication accessory protein and enhances viral replication

Luke A. Selth; Satish C. Dogra; M. Saif Rasheed; Helen Healy; John W. Randles; M. Ali Rezaian

doi:10.1105/tpc.104.027235

A NAC domain protein interacts with Tomato leaf curl virus replication accessory protein and enhances viral replication

Luke A. Selth, Satish C. Dogra, M. Saif Rasheed, Helen Healy, John W. Randles, M. Ali Rezaian

Research output: Contribution to journal › Article › peer-review

178 Citations (Scopus)

Abstract

Geminivirus replication enhancer (REn) proteins dramatically increase the accumulation of viral DNA species by an unknown mechanism. In this study, we present evidence implicating SINAC1, a new member of the NAC domain protein family from tomato (Solanum lycopersicum), in Tomato leaf curl virus (TLCV) REn function. We isolated SINAC1 using yeast (Saccharomyces cerevisiae) two-hybrid technology and TLCV REn as bait, and confirmed the interaction between these proteins in vitro. TLCV induces SINAC1 expression specifically in infected cells, and this upregulation requires REn. In a transient TLCV replication system, overexpression of SINAC1 resulted in a substantial increase in viral DNA accumulation. SINAC1 colocalized with REn to the nucleus and activated transcription of a reporter gene in yeast, suggesting that in healthy cells it functions as a transcription factor. Together, these results imply that SINAC1 plays an important role in the process by which REn enhances TLCV replication.

Original language	English
Pages (from-to)	311-325
Number of pages	15
Journal	Plant Cell
Volume	17
Issue number	1
DOIs	https://doi.org/10.1105/tpc.104.027235
Publication status	Published - 1 Jan 2005
Externally published	Yes

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title = "A NAC domain protein interacts with Tomato leaf curl virus replication accessory protein and enhances viral replication",

abstract = "Geminivirus replication enhancer (REn) proteins dramatically increase the accumulation of viral DNA species by an unknown mechanism. In this study, we present evidence implicating SINAC1, a new member of the NAC domain protein family from tomato (Solanum lycopersicum), in Tomato leaf curl virus (TLCV) REn function. We isolated SINAC1 using yeast (Saccharomyces cerevisiae) two-hybrid technology and TLCV REn as bait, and confirmed the interaction between these proteins in vitro. TLCV induces SINAC1 expression specifically in infected cells, and this upregulation requires REn. In a transient TLCV replication system, overexpression of SINAC1 resulted in a substantial increase in viral DNA accumulation. SINAC1 colocalized with REn to the nucleus and activated transcription of a reporter gene in yeast, suggesting that in healthy cells it functions as a transcription factor. Together, these results imply that SINAC1 plays an important role in the process by which REn enhances TLCV replication.",

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T1 - A NAC domain protein interacts with Tomato leaf curl virus replication accessory protein and enhances viral replication

AU - Selth, Luke A.

AU - Dogra, Satish C.

AU - Rasheed, M. Saif

AU - Healy, Helen

AU - Randles, John W.

AU - Rezaian, M. Ali

PY - 2005/1/1

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N2 - Geminivirus replication enhancer (REn) proteins dramatically increase the accumulation of viral DNA species by an unknown mechanism. In this study, we present evidence implicating SINAC1, a new member of the NAC domain protein family from tomato (Solanum lycopersicum), in Tomato leaf curl virus (TLCV) REn function. We isolated SINAC1 using yeast (Saccharomyces cerevisiae) two-hybrid technology and TLCV REn as bait, and confirmed the interaction between these proteins in vitro. TLCV induces SINAC1 expression specifically in infected cells, and this upregulation requires REn. In a transient TLCV replication system, overexpression of SINAC1 resulted in a substantial increase in viral DNA accumulation. SINAC1 colocalized with REn to the nucleus and activated transcription of a reporter gene in yeast, suggesting that in healthy cells it functions as a transcription factor. Together, these results imply that SINAC1 plays an important role in the process by which REn enhances TLCV replication.

AB - Geminivirus replication enhancer (REn) proteins dramatically increase the accumulation of viral DNA species by an unknown mechanism. In this study, we present evidence implicating SINAC1, a new member of the NAC domain protein family from tomato (Solanum lycopersicum), in Tomato leaf curl virus (TLCV) REn function. We isolated SINAC1 using yeast (Saccharomyces cerevisiae) two-hybrid technology and TLCV REn as bait, and confirmed the interaction between these proteins in vitro. TLCV induces SINAC1 expression specifically in infected cells, and this upregulation requires REn. In a transient TLCV replication system, overexpression of SINAC1 resulted in a substantial increase in viral DNA accumulation. SINAC1 colocalized with REn to the nucleus and activated transcription of a reporter gene in yeast, suggesting that in healthy cells it functions as a transcription factor. Together, these results imply that SINAC1 plays an important role in the process by which REn enhances TLCV replication.

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JO - Plant Cell

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A NAC domain protein interacts with Tomato leaf curl virus replication accessory protein and enhances viral replication

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