A neurofilament protein antibody selectively labels a large ganglion cell type in the human retina

Charles Straznicky; James C. Vickers; Robert Gábriel; Marcello Costa

doi:10.1016/0006-8993(92)90325-4

A neurofilament protein antibody selectively labels a large ganglion cell type in the human retina

Charles Straznicky, James C. Vickers, Robert Gábriel, Marcello Costa

Research output: Contribution to journal › Article › peer-review

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Abstract

An antibody (SMI-32) raised against the non-phosphorylated form of the neurofilament protein triplet (NFP) revealed immunoreactivity in the soma and dendritic arborization of a group of large ganglion cells in the human retina. In addition, a population of smaller somas was also faintly labeled with this antibody in the ganglion cell layer. The completely stained cells amounted to 44,000 and were non-uniformly distributed across the retina with a peak density of 100 cells/mm² in the retinal center to 10 cells/mm² in the retinal periphery. The soma sizes increased about two-fold and dendritic field sizes about 3-fold with retinal eccentricity. The immunoreactive dendrites branched in the vitread sublamina of the inner plexiform layer. The dendritic branching pattern of these cells indicated that they correspond to the previously described shrub cells¹¹. Antibodies against NFP and neuropeptide Y showed colocalization of these markers in all of the completely stained cells.

Original language	English
Pages (from-to)	123-128
Number of pages	6
Journal	Brain Research
Volume	582
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(92)90325-4
Publication status	Published - 5 Jun 1992

Keywords

Colocalization
Human
Large ganglion cell
Neurofilament protein antibody SMI-32
Neuropeptide Y
Retinal distribution
Shrub cell

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title = "A neurofilament protein antibody selectively labels a large ganglion cell type in the human retina",

abstract = "An antibody (SMI-32) raised against the non-phosphorylated form of the neurofilament protein triplet (NFP) revealed immunoreactivity in the soma and dendritic arborization of a group of large ganglion cells in the human retina. In addition, a population of smaller somas was also faintly labeled with this antibody in the ganglion cell layer. The completely stained cells amounted to 44,000 and were non-uniformly distributed across the retina with a peak density of 100 cells/mm2 in the retinal center to 10 cells/mm2 in the retinal periphery. The soma sizes increased about two-fold and dendritic field sizes about 3-fold with retinal eccentricity. The immunoreactive dendrites branched in the vitread sublamina of the inner plexiform layer. The dendritic branching pattern of these cells indicated that they correspond to the previously described shrub cells11. Antibodies against NFP and neuropeptide Y showed colocalization of these markers in all of the completely stained cells.",

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AU - Vickers, James C.

AU - Gábriel, Robert

AU - Costa, Marcello

PY - 1992/6/5

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N2 - An antibody (SMI-32) raised against the non-phosphorylated form of the neurofilament protein triplet (NFP) revealed immunoreactivity in the soma and dendritic arborization of a group of large ganglion cells in the human retina. In addition, a population of smaller somas was also faintly labeled with this antibody in the ganglion cell layer. The completely stained cells amounted to 44,000 and were non-uniformly distributed across the retina with a peak density of 100 cells/mm2 in the retinal center to 10 cells/mm2 in the retinal periphery. The soma sizes increased about two-fold and dendritic field sizes about 3-fold with retinal eccentricity. The immunoreactive dendrites branched in the vitread sublamina of the inner plexiform layer. The dendritic branching pattern of these cells indicated that they correspond to the previously described shrub cells11. Antibodies against NFP and neuropeptide Y showed colocalization of these markers in all of the completely stained cells.

AB - An antibody (SMI-32) raised against the non-phosphorylated form of the neurofilament protein triplet (NFP) revealed immunoreactivity in the soma and dendritic arborization of a group of large ganglion cells in the human retina. In addition, a population of smaller somas was also faintly labeled with this antibody in the ganglion cell layer. The completely stained cells amounted to 44,000 and were non-uniformly distributed across the retina with a peak density of 100 cells/mm2 in the retinal center to 10 cells/mm2 in the retinal periphery. The soma sizes increased about two-fold and dendritic field sizes about 3-fold with retinal eccentricity. The immunoreactive dendrites branched in the vitread sublamina of the inner plexiform layer. The dendritic branching pattern of these cells indicated that they correspond to the previously described shrub cells11. Antibodies against NFP and neuropeptide Y showed colocalization of these markers in all of the completely stained cells.

KW - Colocalization

KW - Human

KW - Large ganglion cell

KW - Neurofilament protein antibody SMI-32

KW - Neuropeptide Y

KW - Retinal distribution

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A neurofilament protein antibody selectively labels a large ganglion cell type in the human retina

Abstract

Keywords

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