A neurofilament protein antibody selectively labels a large ganglion cell type in the human retina

Charles Straznicky, James C. Vickers, Robert Gábriel, Marcello Costa

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    34 Citations (Scopus)

    Abstract

    An antibody (SMI-32) raised against the non-phosphorylated form of the neurofilament protein triplet (NFP) revealed immunoreactivity in the soma and dendritic arborization of a group of large ganglion cells in the human retina. In addition, a population of smaller somas was also faintly labeled with this antibody in the ganglion cell layer. The completely stained cells amounted to 44,000 and were non-uniformly distributed across the retina with a peak density of 100 cells/mm2 in the retinal center to 10 cells/mm2 in the retinal periphery. The soma sizes increased about two-fold and dendritic field sizes about 3-fold with retinal eccentricity. The immunoreactive dendrites branched in the vitread sublamina of the inner plexiform layer. The dendritic branching pattern of these cells indicated that they correspond to the previously described shrub cells11. Antibodies against NFP and neuropeptide Y showed colocalization of these markers in all of the completely stained cells.

    Original languageEnglish
    Pages (from-to)123-128
    Number of pages6
    JournalBrain Research
    Volume582
    Issue number1
    DOIs
    Publication statusPublished - 5 Jun 1992

    Keywords

    • Colocalization
    • Human
    • Large ganglion cell
    • Neurofilament protein antibody SMI-32
    • Neuropeptide Y
    • Retinal distribution
    • Shrub cell

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