A new approach to fecal occult blood testing based on the detection of haptoglobin

Pei Xiang Xing, Graeme P. Young, Deborah Ho, Marc A. Sinatra, Peter B. Hoj, Ian F.C. McKenzie

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

BACKGROUND. Colorectal carcinoma is a common disease, occurring in 1 in 20 adults in Western society, and there is a compelling need for an effective early diagnostic test. Several serum tests, including carcinoembryonic antigen have been used, but none are sufficiently sensitive or specific for the early diagnosis of the disease. METHODS. In a novel approach using fecal extracts from patients with colorectal cancer as the antigen for immunization, several MoAbs were produced. One (FE14.1) was found to react with the feces from patients with colon cancer, but not with those from normal subjects. A sandwich enzyme-linked immunoadsorbent assay was developed, and its ability to diagnose colorectal carcinoma evaluated. RESULTS. Of the patients with colorectal carcinoma, 91.5% (43/46) were positive compared with 1.9% of normal individuals (4/211). Analysis of the N- terminal amino acid sequence of a subunit of the molecule detected by FE14.1 shows it to he the β chain of haptoglobin. CONCLUSIONS. The assay developed in this study has several advantages compared with current fecal occult blood tests, including no requirement for dietary restriction and the ability to distinguish between upper and lower gastrointestinal bleeding, while retaining the sensitivity and specificity of the current tests. Furthermore, the sensitivity of the tests increases to 100% if the FE14.1 and HemeSelect are combined. In addition, the study shows the potential to produce anticancer agents by immunizing with fecal material.

Original languageEnglish
Pages (from-to)48-56
Number of pages9
JournalCancer
Volume78
Issue number1
DOIs
Publication statusPublished - 1 Jul 1996
Externally publishedYes

Keywords

  • colorectal cancer
  • enzyme-linked immunoadsorbent assay
  • feces
  • haptoglobin
  • monoclonal antibodies

Fingerprint Dive into the research topics of 'A new approach to fecal occult blood testing based on the detection of haptoglobin'. Together they form a unique fingerprint.

Cite this