A new cell assay to determine the virulence of Francisella tularensis

K Mack, M Fulop, R J Manchee, C J Stirling

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

A cell culture assay to determine the virulence of Francisella tularensis was devised. Murine cell lines P388 and J774 were significantly more susceptible to F. tularensis Schu4 than the attenuated live vaccine strain. The ability of F. tularensis strains to cause cell death correlated with their virulence to mice. Use of this assay with infected cells separated from susceptible uninfected cells by a membrane with 0.1 μm pores, failed to demonstrate possible diffusible exotoxins produced by F. tularensis.
Original languageEnglish
Pages (from-to)158-160
Number of pages3
JournalLetters in Applied Microbiology
Volume19
Issue number3
DOIs
Publication statusPublished - Sep 1994

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