A new cell assay to determine the virulence of Francisella tularensis

K Mack; M Fulop; R J Manchee; C J Stirling

doi:10.1111/j.1472-765X.1994.tb00931.x

A new cell assay to determine the virulence of Francisella tularensis

K Mack, M Fulop, R J Manchee, C J Stirling

President and Vice-Chancellor

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Abstract

A cell culture assay to determine the virulence of Francisella tularensis was devised. Murine cell lines P388 and J774 were significantly more susceptible to F. tularensis Schu4 than the attenuated live vaccine strain. The ability of F. tularensis strains to cause cell death correlated with their virulence to mice. Use of this assay with infected cells separated from susceptible uninfected cells by a membrane with 0.1 μm pores, failed to demonstrate possible diffusible exotoxins produced by F. tularensis.

Original language	English
Pages (from-to)	158-160
Number of pages	3
Journal	Letters in Applied Microbiology
Volume	19
Issue number	3
DOIs	https://doi.org/10.1111/j.1472-765X.1994.tb00931.x
Publication status	Published - Sept 1994

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title = "A new cell assay to determine the virulence of Francisella tularensis",

abstract = "A cell culture assay to determine the virulence of Francisella tularensis was devised. Murine cell lines P388 and J774 were significantly more susceptible to F. tularensis Schu4 than the attenuated live vaccine strain. The ability of F. tularensis strains to cause cell death correlated with their virulence to mice. Use of this assay with infected cells separated from susceptible uninfected cells by a membrane with 0.1 μm pores, failed to demonstrate possible diffusible exotoxins produced by F. tularensis.",

author = "K Mack and M Fulop and Manchee, {R J} and Stirling, {C J}",

year = "1994",

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AU - Fulop, M

AU - Manchee, R J

AU - Stirling, C J

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Y1 - 1994/9

N2 - A cell culture assay to determine the virulence of Francisella tularensis was devised. Murine cell lines P388 and J774 were significantly more susceptible to F. tularensis Schu4 than the attenuated live vaccine strain. The ability of F. tularensis strains to cause cell death correlated with their virulence to mice. Use of this assay with infected cells separated from susceptible uninfected cells by a membrane with 0.1 μm pores, failed to demonstrate possible diffusible exotoxins produced by F. tularensis.

AB - A cell culture assay to determine the virulence of Francisella tularensis was devised. Murine cell lines P388 and J774 were significantly more susceptible to F. tularensis Schu4 than the attenuated live vaccine strain. The ability of F. tularensis strains to cause cell death correlated with their virulence to mice. Use of this assay with infected cells separated from susceptible uninfected cells by a membrane with 0.1 μm pores, failed to demonstrate possible diffusible exotoxins produced by F. tularensis.

U2 - 10.1111/j.1472-765X.1994.tb00931.x

DO - 10.1111/j.1472-765X.1994.tb00931.x

M3 - Article

SN - 0266-8254

VL - 19

SP - 158

EP - 160

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

IS - 3

ER -

A new cell assay to determine the virulence of Francisella tularensis

Abstract

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