A novel approach for the simultaneous quantification of 18 small molecule kinase inhibitors in human plasma: a novel platform for optimised KI dosing: A platform for optimised KI dosing

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    10 Citations (Scopus)

    Abstract

    Small molecule kinase inhibitors (KIs) are a rapidly expanding class of narrow therapeutic index antineoplastic drugs that exhibit substantial inter-individual variability in exposure. This manuscript describes a novel approach for the quantification of 18 KIs in plasma, providing a platform that is unparalleled in terms of scope for the assessment of KI therapeutic drug monitoring (TDM) and facilitating pharmacokinetic studies with KIs. Following the addition of a panel of four deuterated internal standards, plasma samples were prepared by solvent precipitation with acidified methanol. Analytes were separated on a Waters ACQUITY™ T3 HSS C18 analytical column (150 × 2.1 mm, 1.8 μm particle size) by linear gradient elution, with subsequent detection by time-of-flight mass spectrometry. Time-of-flight data were collected in wide pass mode, with selected ion (pseudo-MRM) spectra extracted at the precursor m/z of analytes in ESI+ mode. The analytical performance of this approach in terms of specificity, linearity, accuracy, precision range, quantification limit and detection limit meet all criteria for an analytical platform for the quantification of drugs. This approach was developed, validated and reported in accordance with the 2015 version of the FDA guidance for industry on ‘analytical procedures are methods validation for drugs and biologics’ facilitating direct application as a clinical trials platform.

    Original languageEnglish
    Pages (from-to)17-26
    Number of pages10
    JournalJournal of Chromatography B-Analytical Technologies in The Biomedical and Life Sciences
    Volume1033-1034
    DOIs
    Publication statusPublished - 15 Oct 2016

    Keywords

    • Pharmacokinetics
    • Small molecule kinase inhibitors
    • Therapeutic drug monitoring
    • Time-of-flight mass spectrometry
    • Ultraperformance liquid chromatography

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