A novel nomenclature for the hypervariable short tandem repeat APOl1

Li Chin Tsai, Kun Long Wu, Hsing Mei Hsieh, Meng Hui Chien, Adrian Linacre, James Chun I. Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A novel nomenclature for the hypervariable microsatellite DNA, APOAI1 locus, is proposed. The complex nature of the repeat unit in this locus results in alleles separated by a single base. Polymerase chain reaction (PCR) products amplified from this locus were separated by single-strand conformation polymorphism (SSCP) electrophoresis. All the single-stranded DNA bands on the SSCP gel were removed from the gel and a second amplification performed. Homozygous DNA fragments amplified from single-stranded DNA were sequenced. From the 100 individuals studied, 30 alleles and 73 genotypes were found. A system of nomenclature for the APOAI1 locus is provided that is logical and in line with previous models. Using the primers described, the locus can be amplified and alleles designated on the basis of size. This system of nomenclature will assist in the exchange of data between laboratories for this locus.

Original languageEnglish
Pages (from-to)1090-1094
Number of pages5
JournalElectrophoresis
Volume22
Issue number6
DOIs
Publication statusPublished - Apr 2001
Externally publishedYes

Keywords

  • APOAl1
  • Polymerase chain reaction
  • Short tandem repeat
  • Single-strand conformation polymorphism

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