A novel red-emitting aggregation-induced emission probe for determination of β-glucosidase activity

Bicheng Yao, Jiamin Zhao, Siyang Ding, Marie Claire Giel, Guoqiang Zhang, Dan Ding, Youhong Tang, Zhe H. Weng, Yuning Hong

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

β-Glucosidase (β-Glu) is a ubiquitous enzyme which has multiple roles in medical diagnosis, food production, agriculture, etc. Existing β-Glu assays have limitations such as complex operation, long running time, and high background noise. Here we report a red-emissive probe TBPG for measuring the activity of β-Glu. The probe was synthesized through conjugating a β-Glu targeting glucoside to an aggregation-induced emission (AIE) fluorophore. In the presence of β-Glu, TBPG was hydrolyzed and exhibited a fluorescence turn-on process. The detection conditions including time, temperature, pH value, buffer, and probe concentration were optimized systematically. Afterwards, fluorescence titration was conducted showing an excellent linearity (R2 = 0.998), a wide linear dynamic range (0–5.0 U/mL), and a limit of detection as low as 0.6 U/L. The detection specificity and ion interference were evaluated by adding various biological species and ions to probe without or with β-Glu. Next, we demonstrate the applicability of probe TBPG in determining the β-Glu activity in living cells using confocal microscopy and flow cytometry. Finally, this newly established assay was applied to real soil samples. Comparable results were obtained as the commercial assay, manifesting its great potential in soil enzyme analysis.

Original languageEnglish
Article number122046
Number of pages11
JournalBiomaterials
Volume295
Early online date11 Feb 2023
DOIs
Publication statusPublished - Apr 2023

Keywords

  • Aggregation-induced emission
  • Enzyme activity probe
  • Fluorescent probes
  • Soil enzyme
  • βGlucosidase

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