TY - JOUR
T1 - A platform for selective immuno-capture of cancer cells from urine
AU - Macgregor-Ramiasa, Melanie
AU - McNicholas, Kym
AU - Ostrikov, Kola
AU - Li, Jordan
AU - Michael, Michael
AU - Gleadle, Jonathan
AU - Vasilev, Krasimir
PY - 2017/10/15
Y1 - 2017/10/15
N2 - Urothelial cancers are amongst the 10 most common types of cancer and represent a major health problem worldwide. Current urinary diagnostic tests for urothelial cancer are expensive and have limited sensitivity and specificity. In this work, proofs of concept for a selective cancer cell capture platform are presented with the aim to achieve the first generation of specific urinary tests for the detection of cancer cells in urine specimen. The unique reactivity of plasma deposited polyoxazoline was used to covalently bind cancer specific antibodies in microchannels. Cancer cells dispersed in patient urine were successfully captured with up to 99% selectivity and 100% sensitivity over a wide range of cell concentrations. The streamlined two steps preparation process of the capture platform represents an important advance in medical diagnostics, with broader potential applications.
AB - Urothelial cancers are amongst the 10 most common types of cancer and represent a major health problem worldwide. Current urinary diagnostic tests for urothelial cancer are expensive and have limited sensitivity and specificity. In this work, proofs of concept for a selective cancer cell capture platform are presented with the aim to achieve the first generation of specific urinary tests for the detection of cancer cells in urine specimen. The unique reactivity of plasma deposited polyoxazoline was used to covalently bind cancer specific antibodies in microchannels. Cancer cells dispersed in patient urine were successfully captured with up to 99% selectivity and 100% sensitivity over a wide range of cell concentrations. The streamlined two steps preparation process of the capture platform represents an important advance in medical diagnostics, with broader potential applications.
KW - Cancer cell capture
KW - Diagnostic devices
KW - Plasma polymers
KW - Polyoxazolines
KW - Urine
UR - http://www.scopus.com/inward/record.url?scp=85018962965&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/NHMRC/1122825
U2 - 10.1016/j.bios.2017.02.011
DO - 10.1016/j.bios.2017.02.011
M3 - Article
C2 - 28479020
SN - 0956-5663
VL - 96
SP - 373
EP - 380
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -