A quick colorimetric method for total lipid quantification in microalgae

Avinesh Byreddy, Adarsha Gupta, Colin Barrow, Munish Puri

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    Discovering microalgae with high lipid productivity are among the key milestones for achieving sustainable biodiesel production. Current methods of lipid quantification are time intensive and costly. A rapid colorimetric method based on sulfo-phospho-vanillin (SPV) reaction was developed for the quantification of microbial lipids to facilitate screening for lipid producing microalgae. This method was successfully tested on marine thraustochytrid strains and vegetable oils. The colorimetric method results correlated well with gravimetric method estimates. The new method was less time consuming than gravimetric analysis and is quantitative for lipid determination, even in the presence of carbohydrates, proteins and glycerol.

    Original languageEnglish
    Pages (from-to)28-32
    Number of pages5
    JournalJournal of Microbiological Methods
    Volume125
    DOIs
    Publication statusPublished - 1 Jun 2016

    Keywords

    • Algae
    • Biodiesel
    • Esterification
    • Industrial biotechnology
    • Vanillin

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