A radioisotopic assay of picomolar concentrations of coenzyme A in liver tissue

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    Abstract

    A single-step enzyme assay using [14C]palmitic acid and bacterial acyl-coenzyme A synthetase (EC 6.2.1.3) is described for the determination of reduced coenzyme A (CoASH) levels in liver samples. Use of this technique provides a rapid and accurate determination of CoASH in the range 1-250 pmol. Application of the method to the quantitation of CoASH in samples of human liver tissue and rat liver homogenate, isolated hepatocytes, and mitochondria is described.

    Original languageEnglish
    Pages (from-to)94-99
    Number of pages6
    JournalAnalytical Biochemistry
    Volume168
    Issue number1
    DOIs
    Publication statusPublished - Jan 1988

    Keywords

    • bioassay
    • coenzyme A
    • hepatocytes
    • human liver
    • liquid scintillation counting
    • mitochondria

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