A radioisotopic assay of picomolar concentrations of coenzyme A in liver tissue

Kathleen M. Knights; Roger Drew

doi:10.1016/0003-2697(88)90015-2

A radioisotopic assay of picomolar concentrations of coenzyme A in liver tissue

Kathleen M. Knights, Roger Drew

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A single-step enzyme assay using [¹⁴C]palmitic acid and bacterial acyl-coenzyme A synthetase (EC 6.2.1.3) is described for the determination of reduced coenzyme A (CoASH) levels in liver samples. Use of this technique provides a rapid and accurate determination of CoASH in the range 1-250 pmol. Application of the method to the quantitation of CoASH in samples of human liver tissue and rat liver homogenate, isolated hepatocytes, and mitochondria is described.

Original language	English
Pages (from-to)	94-99
Number of pages	6
Journal	Analytical Biochemistry
Volume	168
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(88)90015-2
Publication status	Published - Jan 1988

Keywords

bioassay
coenzyme A
hepatocytes
human liver
liquid scintillation counting
mitochondria

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abstract = "A single-step enzyme assay using [14C]palmitic acid and bacterial acyl-coenzyme A synthetase (EC 6.2.1.3) is described for the determination of reduced coenzyme A (CoASH) levels in liver samples. Use of this technique provides a rapid and accurate determination of CoASH in the range 1-250 pmol. Application of the method to the quantitation of CoASH in samples of human liver tissue and rat liver homogenate, isolated hepatocytes, and mitochondria is described.",

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AB - A single-step enzyme assay using [14C]palmitic acid and bacterial acyl-coenzyme A synthetase (EC 6.2.1.3) is described for the determination of reduced coenzyme A (CoASH) levels in liver samples. Use of this technique provides a rapid and accurate determination of CoASH in the range 1-250 pmol. Application of the method to the quantitation of CoASH in samples of human liver tissue and rat liver homogenate, isolated hepatocytes, and mitochondria is described.

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KW - coenzyme A

KW - hepatocytes

KW - human liver

KW - liquid scintillation counting

KW - mitochondria

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ER -

A radioisotopic assay of picomolar concentrations of coenzyme A in liver tissue

Abstract

Keywords

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