A radioisotopic assay of picomolar concentrations of coenzyme A in liver tissue

Kathleen M. Knights; Roger Drew

doi:10.1016/0003-2697(88)90015-2

A radioisotopic assay of picomolar concentrations of coenzyme A in liver tissue

Kathleen M. Knights
, Roger Drew

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A single-step enzyme assay using [¹⁴C]palmitic acid and bacterial acyl-coenzyme A synthetase (EC 6.2.1.3) is described for the determination of reduced coenzyme A (CoASH) levels in liver samples. Use of this technique provides a rapid and accurate determination of CoASH in the range 1-250 pmol. Application of the method to the quantitation of CoASH in samples of human liver tissue and rat liver homogenate, isolated hepatocytes, and mitochondria is described.

Original language	English
Pages (from-to)	94-99
Number of pages	6
Journal	Analytical Biochemistry
Volume	168
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(88)90015-2
Publication status	Published - Jan 1988

Keywords

bioassay
coenzyme A
hepatocytes
human liver
liquid scintillation counting
mitochondria

Access to Document

10.1016/0003-2697(88)90015-2Licence: In Copyright

Cite this

@article{e7dc611b1b2f447e91283b6f06a11103,

title = "A radioisotopic assay of picomolar concentrations of coenzyme A in liver tissue",

abstract = "A single-step enzyme assay using [14C]palmitic acid and bacterial acyl-coenzyme A synthetase (EC 6.2.1.3) is described for the determination of reduced coenzyme A (CoASH) levels in liver samples. Use of this technique provides a rapid and accurate determination of CoASH in the range 1-250 pmol. Application of the method to the quantitation of CoASH in samples of human liver tissue and rat liver homogenate, isolated hepatocytes, and mitochondria is described.",

keywords = "bioassay, coenzyme A, hepatocytes, human liver, liquid scintillation counting, mitochondria",

author = "Knights, \{Kathleen M.\} and Roger Drew",

year = "1988",

month = jan,

doi = "10.1016/0003-2697(88)90015-2",

language = "English",

volume = "168",

pages = "94--99",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - A radioisotopic assay of picomolar concentrations of coenzyme A in liver tissue

AU - Knights, Kathleen M.

AU - Drew, Roger

PY - 1988/1

Y1 - 1988/1

N2 - A single-step enzyme assay using [14C]palmitic acid and bacterial acyl-coenzyme A synthetase (EC 6.2.1.3) is described for the determination of reduced coenzyme A (CoASH) levels in liver samples. Use of this technique provides a rapid and accurate determination of CoASH in the range 1-250 pmol. Application of the method to the quantitation of CoASH in samples of human liver tissue and rat liver homogenate, isolated hepatocytes, and mitochondria is described.

AB - A single-step enzyme assay using [14C]palmitic acid and bacterial acyl-coenzyme A synthetase (EC 6.2.1.3) is described for the determination of reduced coenzyme A (CoASH) levels in liver samples. Use of this technique provides a rapid and accurate determination of CoASH in the range 1-250 pmol. Application of the method to the quantitation of CoASH in samples of human liver tissue and rat liver homogenate, isolated hepatocytes, and mitochondria is described.

KW - bioassay

KW - coenzyme A

KW - hepatocytes

KW - human liver

KW - liquid scintillation counting

KW - mitochondria

UR - https://www.scopus.com/pages/publications/0023818508

U2 - 10.1016/0003-2697(88)90015-2

DO - 10.1016/0003-2697(88)90015-2

M3 - Article

C2 - 3364723

AN - SCOPUS:0023818508

SN - 0003-2697

VL - 168

SP - 94

EP - 99

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

A radioisotopic assay of picomolar concentrations of coenzyme A in liver tissue

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this