A rapid gas-liquid chromatographic determination of antipyrine clearance in the rat

Kathleen M. Knights, Geoffrey K. Gourlay, John F. Adams, Michael J. Cousins

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    The assay procedure described in this paper allows the calculation of pharmacokinetic data of antipyrine disposition to be obtained from a single animal with minimal disturbance in hematocrit. A sample volume (100 μl) allows the repeated use of the same rat for studies of hepatic drug metabolism. The lower limit of sensitivity is 5 μg/ml, with a range of linearity extending to 100 μg/ml. This allows for various dosing regimens. The GLC assay is rapid and accurate as shown by comparison with previously published values for the half-life of antipyrine in rats.

    Original languageEnglish
    Pages (from-to)121-125
    Number of pages5
    JournalJournal of Pharmacological Methods
    Volume5
    Issue number2
    DOIs
    Publication statusPublished - Mar 1981

    Keywords

    • Antipyrine analysis
    • Gas liquid chromatography
    • Rat
    • Whole blood

    Fingerprint Dive into the research topics of 'A rapid gas-liquid chromatographic determination of antipyrine clearance in the rat'. Together they form a unique fingerprint.

  • Cite this