A rapid wire based sampling method for DNA profiling

Tong Chen, David Catcheside, Alice Stephenson, Chris Hefford, Kenneth Kirkbride, Leigh Burgoyne

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    This paper reports the results of a commission to develop a field deployable rapid short tandem repeat (STR)-based DNA profiling system to enable discrimination between tissues derived from a small number of individuals. Speed was achieved by truncation of sample preparation and field deployability by use of an Agilent 2100 Bioanalyser TM. Human blood and tissues were stabbed with heated stainless steel wire and the resulting sample dehydrated with isopropanol prior to direct addition to a PCR. Choice of a polymerase tolerant of tissue residues and cycles of amplification appropriate for the amount of template expected yielded useful profiles with a custom-designed quintuplex primer set suitable for use with the Bioanalyser TM. Samples stored on wires remained amplifiable for months, allowing their transportation unrefrigerated from remote locations to a laboratory for analysis using AmpFlSTR ® Profiler Plus ® without further processing. The field system meets the requirements for discrimination of samples from small sets and retains access to full STR profiling when required.

    Original languageEnglish
    Pages (from-to)472-477
    Number of pages6
    JournalJournal of Forensic Sciences
    Volume57
    Issue number2
    DOIs
    Publication statusPublished - Mar 2012

    Keywords

    • Biological sampling
    • Disaster victim identification
    • DNA profiling
    • Forensic science
    • Stainless steel wire

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