A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma

Brett W. Stringer; Bryan W. Day; Rochelle C.J. D’Souza; Paul R. Jamieson; Kathleen S. Ensbey; Zara C. Bruce; Yi Chieh Lim; Kate Goasdoué; Carolin Offenhäuser; Seçkin Akgül; Suzanne Allan; Thomas Robertson; Peter Lucas; Gert Tollesson; Scott Campbell; Craig Winter; Hongdo Do; Alexander Dobrovic; Po Ling Inglis; Rosalind L. Jeffree; Terrance G. Johns; Andrew W. Boyd

doi:10.1038/s41598-019-41277-z

A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma

Brett W. Stringer, Bryan W. Day, Rochelle C.J. D’Souza, Paul R. Jamieson, Kathleen S. Ensbey, Zara C. Bruce, Yi Chieh Lim, Kate Goasdoué, Carolin Offenhäuser, Seçkin Akgül, Suzanne Allan, Thomas Robertson, Peter Lucas, Gert Tollesson, Scott Campbell, Craig Winter, Hongdo Do, Alexander Dobrovic, Po Ling Inglis, Rosalind L. JeffreeTerrance G. Johns, Andrew W. Boyd

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Abstract

Low-passage, serum-free cell lines cultured from patient tumour tissue are the gold-standard for preclinical studies and cellular investigations of glioblastoma (GBM) biology, yet entrenched, poorly-representative cell line models are still widely used, compromising the significance of much GBM research. We submit that greater adoption of these critical resources will be promoted by the provision of a suitably-sized, meaningfully-described reference collection along with appropriate tools for working with them. Consequently, we present a curated panel of 12 readily-usable, genetically-diverse, tumourigenic, patient-derived, low-passage, serum-free cell lines representing the spectrum of molecular subtypes of IDH-wildtype GBM along with their detailed phenotypic characterisation plus a bespoke set of lentiviral plasmids for bioluminescent/fluorescent labelling, gene expression and CRISPR/Cas9-mediated gene inactivation. The cell lines and all accompanying data are readily-accessible via a single website, Q-Cell (qimrberghofer.edu.au/q-cell/) and all plasmids are available from Addgene. These resources should prove valuable to investigators seeking readily-usable, well-characterised, clinically-relevant, gold-standard models of GBM.

Original language	English
Article number	4902
Number of pages	14
Journal	Scientific Reports
Volume	9
DOIs	https://doi.org/10.1038/s41598-019-41277-z
Publication status	Published - Dec 2019
Externally published	Yes

Bibliographical note

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Keywords

molecular targets
glioblastoma
xenografts

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10.1038/s41598-019-41277-zLicence: CC BY

Stringer_reference_P2019Final published version, 3.76 MBLicence: CC BY

Cite this

Stringer, B. W., Day, B. W., D’Souza, R. C. J., Jamieson, P. R., Ensbey, K. S., Bruce, Z. C., Lim, Y. C., Goasdoué, K., Offenhäuser, C., Akgül, S., Allan, S., Robertson, T., Lucas, P., Tollesson, G., Campbell, S., Winter, C., Do, H., Dobrovic, A., Inglis, P. L., ... Boyd, A. W. (2019). A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma. Scientific Reports, 9, Article 4902. https://doi.org/10.1038/s41598-019-41277-z

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abstract = "Low-passage, serum-free cell lines cultured from patient tumour tissue are the gold-standard for preclinical studies and cellular investigations of glioblastoma (GBM) biology, yet entrenched, poorly-representative cell line models are still widely used, compromising the significance of much GBM research. We submit that greater adoption of these critical resources will be promoted by the provision of a suitably-sized, meaningfully-described reference collection along with appropriate tools for working with them. Consequently, we present a curated panel of 12 readily-usable, genetically-diverse, tumourigenic, patient-derived, low-passage, serum-free cell lines representing the spectrum of molecular subtypes of IDH-wildtype GBM along with their detailed phenotypic characterisation plus a bespoke set of lentiviral plasmids for bioluminescent/fluorescent labelling, gene expression and CRISPR/Cas9-mediated gene inactivation. The cell lines and all accompanying data are readily-accessible via a single website, Q-Cell (qimrberghofer.edu.au/q-cell/) and all plasmids are available from Addgene. These resources should prove valuable to investigators seeking readily-usable, well-characterised, clinically-relevant, gold-standard models of GBM.",

keywords = "molecular targets, glioblastoma, xenografts",

author = "Stringer, {Brett W.} and Day, {Bryan W.} and D{\textquoteright}Souza, {Rochelle C.J.} and Jamieson, {Paul R.} and Ensbey, {Kathleen S.} and Bruce, {Zara C.} and Lim, {Yi Chieh} and Kate Goasdou{\'e} and Carolin Offenh{\"a}user and Se{\c c}kin Akg{\"u}l and Suzanne Allan and Thomas Robertson and Peter Lucas and Gert Tollesson and Scott Campbell and Craig Winter and Hongdo Do and Alexander Dobrovic and Inglis, {Po Ling} and Jeffree, {Rosalind L.} and Johns, {Terrance G.} and Boyd, {Andrew W.}",

note = "Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article{\textquoteright}s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article{\textquoteright}s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.",

year = "2019",

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doi = "10.1038/s41598-019-41277-z",

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Stringer, BW, Day, BW, D’Souza, RCJ, Jamieson, PR, Ensbey, KS, Bruce, ZC, Lim, YC, Goasdoué, K, Offenhäuser, C, Akgül, S, Allan, S, Robertson, T, Lucas, P, Tollesson, G, Campbell, S, Winter, C, Do, H, Dobrovic, A, Inglis, PL, Jeffree, RL, Johns, TG & Boyd, AW 2019, 'A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma', Scientific Reports, vol. 9, 4902. https://doi.org/10.1038/s41598-019-41277-z

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AU - D’Souza, Rochelle C.J.

AU - Jamieson, Paul R.

AU - Ensbey, Kathleen S.

AU - Bruce, Zara C.

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AU - Akgül, Seçkin

AU - Allan, Suzanne

AU - Robertson, Thomas

AU - Lucas, Peter

AU - Tollesson, Gert

AU - Campbell, Scott

AU - Winter, Craig

AU - Do, Hongdo

AU - Dobrovic, Alexander

AU - Inglis, Po Ling

AU - Jeffree, Rosalind L.

AU - Johns, Terrance G.

AU - Boyd, Andrew W.

N1 - Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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N2 - Low-passage, serum-free cell lines cultured from patient tumour tissue are the gold-standard for preclinical studies and cellular investigations of glioblastoma (GBM) biology, yet entrenched, poorly-representative cell line models are still widely used, compromising the significance of much GBM research. We submit that greater adoption of these critical resources will be promoted by the provision of a suitably-sized, meaningfully-described reference collection along with appropriate tools for working with them. Consequently, we present a curated panel of 12 readily-usable, genetically-diverse, tumourigenic, patient-derived, low-passage, serum-free cell lines representing the spectrum of molecular subtypes of IDH-wildtype GBM along with their detailed phenotypic characterisation plus a bespoke set of lentiviral plasmids for bioluminescent/fluorescent labelling, gene expression and CRISPR/Cas9-mediated gene inactivation. The cell lines and all accompanying data are readily-accessible via a single website, Q-Cell (qimrberghofer.edu.au/q-cell/) and all plasmids are available from Addgene. These resources should prove valuable to investigators seeking readily-usable, well-characterised, clinically-relevant, gold-standard models of GBM.

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A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma

Abstract

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Keywords

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