A reporter system for replication-competent gammaretroviruses: the inGluc-MLV-DERSE assay

Amanda Aloia, Lisa Duffy, Vladimir Pak, KyeongEun Lee, Silvia Sanchez-Martinez, David Dearse, Gisela Heidecker, Kenneth Cornetta, Alan Rein

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    6 Citations (Scopus)

    Abstract

    Although novel retroviral vectors for use in gene-therapy products are reducing the potential for formation of replication-competent retrovirus (RCR), it remains crucial to screen products for RCR for both research and clinical purposes. For clinical-grade gammaretrovirus-based vectors, RCR screening is achieved by an extended S+ L- or marker-rescue assay, whereas standard methods for replication-competent lentivirus detection are still in development. In this report, we describe a rapid and sensitive method for replication-competent gammaretrovirus detection. We used this assay to detect three members of the gammaretrovirus family and compared the sensitivity of our assay with well-established methods for retrovirus detection, including the extended S+ L- assay. Results presented here demonstrate that this assay should be useful for gene-therapy product testing.

    Original languageEnglish
    Pages (from-to)169-176
    Number of pages8
    JournalGene Therapy
    Volume20
    Issue number2
    DOIs
    Publication statusPublished - Feb 2013

    Keywords

    • Gene-therapy vectors
    • Infectivity assay
    • Replication-competent gammaretrovirus

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  • Cite this

    Aloia, A., Duffy, L., Pak, V., Lee, K., Sanchez-Martinez, S., Dearse, D., Heidecker, G., Cornetta, K., & Rein, A. (2013). A reporter system for replication-competent gammaretroviruses: the inGluc-MLV-DERSE assay. Gene Therapy, 20(2), 169-176. https://doi.org/10.1038/gt.2012.18