Abstract
A sensitive, kinetic assay for UDPglucuronosyltransferase has been devised using 1-naphthol as substrate. It is based on the continuous fluorometric monitoring of 1-naphthol glucuronide production in the cuvette during the reaction. Interference in the measurement of glucuronide fluorescence by 1-naphthol is negligible, since both aglycone and glucuronide have widely differing fluorescence characteristics in the reaction mixture. This method is as sensitive as other assays using 1-naphthol as substrate but has the added advantage that no extraction procedures are involved and the course of the enzymic reaction can be followed directly.
Original language | English |
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Pages (from-to) | 362-368 |
Number of pages | 7 |
Journal | Analytical Biochemistry |
Volume | 109 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1 Jan 1980 |
Externally published | Yes |