A sensitive kinetic assay for UDPGlucuronosyltransferase using l-naphthol as substrate

Peter I. Mackenzie, Osmo Hänninen

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

A sensitive, kinetic assay for UDPglucuronosyltransferase has been devised using 1-naphthol as substrate. It is based on the continuous fluorometric monitoring of 1-naphthol glucuronide production in the cuvette during the reaction. Interference in the measurement of glucuronide fluorescence by 1-naphthol is negligible, since both aglycone and glucuronide have widely differing fluorescence characteristics in the reaction mixture. This method is as sensitive as other assays using 1-naphthol as substrate but has the added advantage that no extraction procedures are involved and the course of the enzymic reaction can be followed directly.

Original languageEnglish
Pages (from-to)362-368
Number of pages7
JournalAnalytical Biochemistry
Volume109
Issue number2
DOIs
Publication statusPublished - 1 Jan 1980
Externally publishedYes

Fingerprint Dive into the research topics of 'A sensitive kinetic assay for UDPGlucuronosyltransferase using l-naphthol as substrate'. Together they form a unique fingerprint.

  • Cite this