A sensitive kinetic assay for UDPGlucuronosyltransferase using l-naphthol as substrate

Peter I. Mackenzie; Osmo Hänninen

doi:10.1016/0003-2697(80)90661-2

A sensitive kinetic assay for UDPGlucuronosyltransferase using l-naphthol as substrate

Peter I. Mackenzie, Osmo Hänninen

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Abstract

A sensitive, kinetic assay for UDPglucuronosyltransferase has been devised using 1-naphthol as substrate. It is based on the continuous fluorometric monitoring of 1-naphthol glucuronide production in the cuvette during the reaction. Interference in the measurement of glucuronide fluorescence by 1-naphthol is negligible, since both aglycone and glucuronide have widely differing fluorescence characteristics in the reaction mixture. This method is as sensitive as other assays using 1-naphthol as substrate but has the added advantage that no extraction procedures are involved and the course of the enzymic reaction can be followed directly.

Original language	English
Pages (from-to)	362-368
Number of pages	7
Journal	Analytical Biochemistry
Volume	109
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(80)90661-2
Publication status	Published - 1 Jan 1980
Externally published	Yes

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AB - A sensitive, kinetic assay for UDPglucuronosyltransferase has been devised using 1-naphthol as substrate. It is based on the continuous fluorometric monitoring of 1-naphthol glucuronide production in the cuvette during the reaction. Interference in the measurement of glucuronide fluorescence by 1-naphthol is negligible, since both aglycone and glucuronide have widely differing fluorescence characteristics in the reaction mixture. This method is as sensitive as other assays using 1-naphthol as substrate but has the added advantage that no extraction procedures are involved and the course of the enzymic reaction can be followed directly.

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A sensitive kinetic assay for UDPGlucuronosyltransferase using l-naphthol as substrate

Abstract

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