A Simple Approach to Patterned Protein Immobilization on Silicon via Electrografting from Diazonium Salt Solutions

Benjamin Flavel, Andrew Gross, David Garrett, Volker Nock, Alison Downard

    Research output: Contribution to journalArticle

    44 Citations (Scopus)

    Abstract

    A highly versatile method utilizing diazonium salt chemistry has been developed for the fabrication of protein arrays. Conventional ultraviolet mask lithography was used to pattern micrometer sized regions into a commercial photoresist on a highly doped p-type silicon (100) substrate. These patterned regions were used as a template for the electrochemical grafting of the in situ generated p-aminobenzenediazonium cation to form patterns of aminophenyl film on silicon. Immobilization of biomolecules was demonstrated by coupling biotin to the aminophenyl regions followed by reaction with fluorescently labeled avidin and visualization with fluorescence microscopy. This simple patterning strategy is promising for future application in biosensor devices.

    Original languageEnglish
    Pages (from-to)1184-1190
    Number of pages7
    JournalACS Applied Materials & Interfaces
    Volume2
    Issue number4
    DOIs
    Publication statusPublished - 28 Apr 2010

    Keywords

    • Avidin
    • Biotin
    • Electrochemical
    • P-phenylenediamine
    • Pattern

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