A tethered bilayer lipid membrane that mimics microbial membranes

Jakob Andersson, Melanie A. Fuller, Kathleen Wood, Stephen A. Holt, Ingo Köper

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    A model membrane system has been developed, which mimics the outer membrane of Gram negative bacteria. The structure is based on a tethered monolayer which has been fused with vesicles containing lipopolysaccharide molecules. The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated. By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane. The influence of divalent ions on the membrane stability has been probed as well as the interaction of the bilayer with the antibiotic colistin. A number of different architectures were developed, suited to both the study of bacterial membrane proteins and the screening of antimicrobial activity of potential drug candidates.

    Original languageEnglish
    Pages (from-to)12958-12969
    Number of pages12
    JournalPhysical Chemistry Chemical Physics
    Volume20
    Issue number18
    DOIs
    Publication statusPublished - 28 May 2018

    Keywords

    • model membrane system
    • Gram negative bacteria
    • lipopolysaccharide molecules

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