A truncated glycoprotein G vaccine formulated with Advax-CpG adjuvant provides protection of mice against genital herpes simplex virus 2 infection

Staffan Görander, Yoshikazu Honda-Okubo, Malin Bäckström, Jeremy Baldwin, Tomas Bergström, Nikolai Petrovsky, Jan Åke Liljeqvist

Research output: Contribution to journalArticlepeer-review

Abstract

Herpes simplex virus type 2 (HSV-2) is a common sexually transmitted disease that affects approximately 500 million individuals globally. There is currently no approved vaccine to prevent HSV-2 infection. EXCT4 is a truncated form of the mature glycoprotein G-2 (mgG-2) that unlike full mature form is secreted by expressing cells enabling it to be rapidly scaled up for production. The current study examined whether EXCT4 immunity in mice could be further enhanced through use of adjuvants. EXCT4 formulated with Advax-CpG adjuvant induced a strong Th1-type immune response characterized by interferon gamma (IFN-γ) and protected animals against a lethal genital challenge with HSV-2. This response was associated with reduced viral load in vaginal washes, spinal cord, and dorsal root ganglia. Together the results provide proof of concept that EXCT4 formulated with Advax-CpG adjuvant is a promising HSV-2 vaccine candidate warranting further investigation.

Original languageEnglish
Pages (from-to)5866-5875
Number of pages10
JournalVaccine
Volume39
Issue number40
DOIs
Publication statusPublished - 24 Sep 2021

Keywords

  • Adjuvant
  • Advax
  • CpG
  • Herpes simplex virus 2
  • HSV-2
  • TLR-9
  • Vaccine

Fingerprint

Dive into the research topics of 'A truncated glycoprotein G vaccine formulated with Advax-CpG adjuvant provides protection of mice against genital herpes simplex virus 2 infection'. Together they form a unique fingerprint.

Cite this