Aberrant mucin glycoprotein patterns of chronic rhinosinusitis patients with bacterial biofilms

Lor Wai Tan, Alkis Psaltis, Leonie Baker, Michael McGuckin, Karine Rousseau, Peter Wormald

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    Background: Increasingly bacterial biofilms have been implicated in chronic rhinosinusitis (CRS), and conventional treatment methods have failed to completely eradicate biofilms. (1) Terminal sialic acids present on sinus mucosal glycoproteins are targets for bacterial adherence and biofilm formation. (2) A subpopulation of CRS patients is more susceptible to biofilm formation due to aberrant terminal sialic residue distribution patterns of glycoproteins on their mucosa. (3) The higher levels of sialyl transferase (ST)6Gal1 gene expression contribute to the overall aberrant glycosylation patterns on the host mucosa that predispose this patient cohort to developing biofilms. (4) Mucin glycoprotein MUC7 that has known bactericidal activity displays an overall reduced terminal sugar profile in biofilm positive CRS patients. Methods: Confocal scanning laser microscopy, glycoarray analysis, real-time polymerase chain reaction of ST6Gal1, neuraminidase assays and multivariate analysis were used to compare production of sialic acid - degrading enzymes in sinus biopsies from biofilm positive and negative CRS patients with mucosa from healthy controls. Results: Biofilm-positive CRS patients expressed aberrant glycoprotein patterns with terminal sialics of between 70 and 90 kD (stress value = 0.1414). The ST6Gal1 gene was upregulated, and bacteria isolated from these patients exhibit significantly higher neuraminidase activity (p = 0.0343). We detected a significant lack in the overall expression of terminal sugar residues of MUC7 (stress value = 0.088). Conclusions: We observed a strong positive correlation between the aberrant terminal sugar patterns in this sub group of CRS patients with biofilms. The innate immunity function of their MUC7 glycoprotein against bacterial invasion may be compromised in CRS patients.

    Original languageEnglish
    Pages (from-to)319-324
    Number of pages6
    JournalAmerican Journal of Rhinology & Allergy
    Volume24
    Issue number5
    DOIs
    Publication statusPublished - Sep 2010

    Fingerprint Dive into the research topics of 'Aberrant mucin glycoprotein patterns of chronic rhinosinusitis patients with bacterial biofilms'. Together they form a unique fingerprint.

    Cite this