Abietane diterpenoids synthesized by suspension-cultured cells of Cephalotaxus fortunei

Xiao-Hui Xu, Wei Zhang, Xu-Peng Cao, Song Xue

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    Cell suspension cultures of Cephalotaxus fortunei were manipulated to produce secondary metabolites of pharmaceutical interest. Seven abietane diterpenoids (six known and one new) were produced by the suspension-cultured C. fortunei cells by complementing an elicitation strategy with an in situ product removal strategy. Based on the results of spectrometric analysis, the structure of the new compound was determined to be 20(10→5)-abeo-4,5-seco-5(10),6,8, 11,13-abietapentaene-3-one (1). The six known compounds were identified as 12-hydroxyabieta-6,8,11,13-tetraene-3-one (2), abieta-8,11,13-triene-3β,12- diol (hinokiol) (3), abietatriene-3β-ol (4), 11,12-dihydroxy-8,11,13- abietatriene-3,7-dione (5), 11-hydroxy-12-methoxyabieta-8,11,13-triene-3,7-dione (6), and 3β,11-dihydroxy-12-methoxyabieta-8,11,13-triene-7-one (7). This is the first time that diterpenoids have been isolated and identified from this pharmaceutically important plant. These results suggest that the strategies we applied were effective methods for synthesizing abietane diterpenoids via a suspension culture of C. fortunei cells.

    Original languageEnglish
    Pages (from-to)52-55
    Number of pages4
    JournalPhytochemistry Letters
    Volume4
    Issue number1
    DOIs
    Publication statusPublished - 15 Mar 2011

    Keywords

    • Abietane diterpenoids
    • Cell suspension culture
    • Cephalotaxus fortunei
    • Elicitation
    • In situ product removal

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