Accelerating Enzymatic Catalysis Using Vortex Fluidics

Joshua Britton, Luz Meneghini, Colin Raston, Gregory Weiss

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    Enzymes catalyze chemical transformations with outstanding stereo- and regio-specificities, but many enzymes are limited by their long reaction times. A general method to accelerate enzymes using pressure waves contained within thin films is described. Each enzyme responds best to specific frequencies of pressure waves, and an acceleration landscape for each protein is reported. A vortex fluidic device introduces pressure waves that drive increased rate constants (kcat) and enzymatic efficiency (kcat/Km). Four enzymes displayed an average seven-fold acceleration, with deoxyribose-5-phosphate aldolase (DERA) achieving an average 15-fold enhancement using this approach. In solving a common problem in enzyme catalysis, a powerful, generalizable tool for enzyme acceleration has been uncovered. This research provides new insights into previously uncontrolled factors affecting enzyme function.

    Original languageEnglish
    Pages (from-to)11387-11391
    Number of pages5
    JournalAngewandte Chemie-International Edition
    Volume55
    Issue number38
    DOIs
    Publication statusPublished - 2016

    Keywords

    • aldolases
    • biocatalysis
    • enzyme acceleration
    • hydrolases
    • vortex fluidics

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