Accumulation of effector memory CD8+ T cells in nasal polyps

Harshita Pant, Amy Hughes, Dijana Miljkovic, Mark Schembri, Peter Wormald, Peta Macardle, Randall Grose, Heddy Zola, Doreen Krumbiegel

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    16 Citations (Scopus)

    Abstract

    Background: T lymphocytes are prevalent in sinus mucosa and are implicated in chronic rhinosinusitis (CRS) pathogenesis. However, the major T-cell subpopulations, helper (CD4+) and cytotoxic (CD8+), have not been adequately examined in CRS. This study was designed to characterize human sinus mucosa and peripheral blood (PB) CD4+ and CD8+ T cells and their level of differentiation in CRS with nasal polyps (NPs), CRS without NPs, and control patients. Methods: A prospective study was performed. Percentages of CD4+ and CD8+ T cells and their levels of differentiation were analyzed in sinus mucosa and PB by flow cytometry. Cell populations were defined as naive, central memory, effector memory, and effector T cells using cell surface markers CD45RA, CD62L, and CD27. The influence of coexisting allergy, sinus eosinophilic mucus (EM), and culture results were examined. Results: In all patients, sinus mucosa had a lower percentage of CD4+ and a higher percentage of CD8+ T cells compared with PB. However, CRS with NPs (n = 86) had a significantly higher percentage of mucosal CD8+ T cells compared with CRS without NPs (n = 40) in control (n = 13) patients (p < 0.0001). Effector memory T cells were increased in sinuses compared with PB in all patients; however, the percentage of effector memory CD8+ T cells was greatest in CRS with NP mucosa (p = 0.002). Surprisingly coexisting allergy or culture results did not influence the mucosal T-cell phenotype. CRS with NP patients with sinus EM had a significantly higher percentage of mucosal CD8+ T cells. Conclusion: Sinus mucosa in CRS with NPs is characterized by a significant enrichment of CD8+ T cells and a relative deficiency of CD4+ T cells. The majority of NP CD8+ T cells had a terminally differentiated, mature, effector memory phenotype, which raises the question, whether these cells are pathogenic or appear as a consequence of inflammation, independent of the presence of allergy or positive microbial culture.

    Original languageEnglish
    Pages (from-to)e117-e126
    Number of pages10
    JournalAmerican Journal of Rhinology & Allergy
    Volume27
    Issue number5
    DOIs
    Publication statusPublished - Sep 2013

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    Pant, H., Hughes, A., Miljkovic, D., Schembri, M., Wormald, P., Macardle, P., Grose, R., Zola, H., & Krumbiegel, D. (2013). Accumulation of effector memory CD8+ T cells in nasal polyps. American Journal of Rhinology & Allergy, 27(5), e117-e126. https://doi.org/10.2500/ajra.2013.27.3958