Accurate detection of methylated cytosine in complex methylation landscapes.

Ramkumar Palanisamy; Ashley Connolly; Matt Trau

doi:10.1021/ac3031948

Accurate detection of methylated cytosine in complex methylation landscapes.

Ramkumar Palanisamy, Ashley Connolly, Matt Trau

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Monitoring DNA methylation can be a useful biomarker for disease diagnosis and prognosis. However, monitoring the methylation status of a specific cytosine biomarker is often confounded by heterogeneous peripheral DNA methylation. To address this issue, molecular inversion probes were designed with inosine strategically positioned to complement suspected DNA methylation sites. This enabled the methylation status of a specific cytosine to be accurately measured with a high level of specificity, irrespective of adjacent epigenetic modifications.

Original language	English
Pages (from-to)	6575-6579
Number of pages	5
Journal	Analytical Chemistry
Volume	85
Issue number	14
DOIs	https://doi.org/10.1021/ac3031948
Publication status	Published - 16 Jul 2013

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abstract = "Monitoring DNA methylation can be a useful biomarker for disease diagnosis and prognosis. However, monitoring the methylation status of a specific cytosine biomarker is often confounded by heterogeneous peripheral DNA methylation. To address this issue, molecular inversion probes were designed with inosine strategically positioned to complement suspected DNA methylation sites. This enabled the methylation status of a specific cytosine to be accurately measured with a high level of specificity, irrespective of adjacent epigenetic modifications.",

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AB - Monitoring DNA methylation can be a useful biomarker for disease diagnosis and prognosis. However, monitoring the methylation status of a specific cytosine biomarker is often confounded by heterogeneous peripheral DNA methylation. To address this issue, molecular inversion probes were designed with inosine strategically positioned to complement suspected DNA methylation sites. This enabled the methylation status of a specific cytosine to be accurately measured with a high level of specificity, irrespective of adjacent epigenetic modifications.

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Accurate detection of methylated cytosine in complex methylation landscapes.

Abstract

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