Acquisition and role of molybdate in Pseudomonas aeruginosa

Victoria G. Pederick, Bart A. Eijkelkamp, Miranda P. Ween, Stephanie L. Begg, James C. Paton, Christopher A. McDevitt

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18 Citations (Scopus)

Abstract

In microaerophilic or anaerobic environments, Pseudomonas aeruginosa utilizes nitrate reduction for energy production, a process dependent on the availability of the oxyanionic form of molybdenum, molybdate (MoO4 2-). Here, we show that molybdate acquisition in P. aeruginosa occurs via a high-affinity ATP-binding cassette permease (ModABC). ModA is a cluster D-III solute binding protein capable of interacting with molybdate or tungstate oxyanions. Deletion of the modA gene reduces cellular molybdate concentrations and results in inhibition of anaerobic growth and nitrate reduction. Further, we show that conditions that permit nitrate reduction also cause inhibition of biofilm formation and an alteration in fatty acid composition of P. aeruginosa. Collectively, these data highlight the importance of molybdate for anaerobic growth of P. aeruginosa and reveal novel consequences of nitrate reduction on biofilm formation and cell membrane composition.

Original languageEnglish
Pages (from-to)6843-6852
Number of pages10
JournalApplied and Environmental Microbiology
Volume80
Issue number21
Early online date6 Oct 2014
DOIs
Publication statusPublished - Nov 2014
Externally publishedYes

Keywords

  • Pseudomonas aeruginosa
  • molybdenum
  • ModABC

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    Pederick, V. G., Eijkelkamp, B. A., Ween, M. P., Begg, S. L., Paton, J. C., & McDevitt, C. A. (2014). Acquisition and role of molybdate in Pseudomonas aeruginosa. Applied and Environmental Microbiology, 80(21), 6843-6852. https://doi.org/10.1128/AEM.02465-14