Addition of magnesium chloride to enhance mono-dispersity of a coiled-coil recombinant mouse macrophage protein

Parveen Pahuja, Alagiri Srinivasan, Munish Puri

    Research output: Contribution to journalArticle

    Abstract

    X-ray crystallography for the determination of three-dimensional structures of protein macromolecules represents an important tool in function assignment of uncharacterized proteins. However, crystallisation is often difficult to achieve. A protein sample fully characterized in terms of dispersity may increase the likelihood of successful crystallisation by improving the predictability of the crystallisation process. To maximize the probability of crystallisation of a novel mouse macrophage protein (rMMP), target molecule was characterized and refined to improve monodispersity. Addition of MgCl 2 at low concentrations resolves the rMMP into a monodisperse solution, and finally successful crystallization of rMMP was achieved. The effect of MgCl2 was studied using gel filtration chromatography and dynamic light scattering.

    Original languageEnglish
    Pages (from-to)133-139
    Number of pages7
    JournalMOLECULAR AND CELLULAR BIOCHEMISTRY
    Volume389
    Issue number1-2
    DOIs
    Publication statusPublished - Apr 2014

    Keywords

    • Crystallisation
    • Dispersity
    • Dynamic light scattering
    • Gel filtration chromatography

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