Addition of magnesium chloride to enhance mono-dispersity of a coiled-coil recombinant mouse macrophage protein

Parveen Pahuja; Alagiri Srinivasan; Munish Puri

doi:10.1007/s11010-013-1934-x

Addition of magnesium chloride to enhance mono-dispersity of a coiled-coil recombinant mouse macrophage protein

Parveen Pahuja, Alagiri Srinivasan, Munish Puri

Research output: Contribution to journal › Article › peer-review

Abstract

X-ray crystallography for the determination of three-dimensional structures of protein macromolecules represents an important tool in function assignment of uncharacterized proteins. However, crystallisation is often difficult to achieve. A protein sample fully characterized in terms of dispersity may increase the likelihood of successful crystallisation by improving the predictability of the crystallisation process. To maximize the probability of crystallisation of a novel mouse macrophage protein (rMMP), target molecule was characterized and refined to improve monodispersity. Addition of MgCl ₂ at low concentrations resolves the rMMP into a monodisperse solution, and finally successful crystallization of rMMP was achieved. The effect of MgCl₂ was studied using gel filtration chromatography and dynamic light scattering.

Original language	English
Pages (from-to)	133-139
Number of pages	7
Journal	MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume	389
Issue number	1-2
DOIs	https://doi.org/10.1007/s11010-013-1934-x
Publication status	Published - Apr 2014

Keywords

Crystallisation
Dispersity
Dynamic light scattering
Gel filtration chromatography

Access to Document

10.1007/s11010-013-1934-xLicence: In Copyright

Cite this

@article{445deb6d67624e4b9a146aa95cb5c9e1,

title = "Addition of magnesium chloride to enhance mono-dispersity of a coiled-coil recombinant mouse macrophage protein",

abstract = "X-ray crystallography for the determination of three-dimensional structures of protein macromolecules represents an important tool in function assignment of uncharacterized proteins. However, crystallisation is often difficult to achieve. A protein sample fully characterized in terms of dispersity may increase the likelihood of successful crystallisation by improving the predictability of the crystallisation process. To maximize the probability of crystallisation of a novel mouse macrophage protein (rMMP), target molecule was characterized and refined to improve monodispersity. Addition of MgCl 2 at low concentrations resolves the rMMP into a monodisperse solution, and finally successful crystallization of rMMP was achieved. The effect of MgCl2 was studied using gel filtration chromatography and dynamic light scattering.",

keywords = "Crystallisation, Dispersity, Dynamic light scattering, Gel filtration chromatography",

author = "Parveen Pahuja and Alagiri Srinivasan and Munish Puri",

year = "2014",

month = apr,

doi = "10.1007/s11010-013-1934-x",

language = "English",

volume = "389",

pages = "133--139",

journal = "MOLECULAR AND CELLULAR BIOCHEMISTRY",

issn = "0300-8177",

publisher = "Springer Netherlands",

number = "1-2",

}

TY - JOUR

T1 - Addition of magnesium chloride to enhance mono-dispersity of a coiled-coil recombinant mouse macrophage protein

AU - Pahuja, Parveen

AU - Srinivasan, Alagiri

AU - Puri, Munish

PY - 2014/4

Y1 - 2014/4

N2 - X-ray crystallography for the determination of three-dimensional structures of protein macromolecules represents an important tool in function assignment of uncharacterized proteins. However, crystallisation is often difficult to achieve. A protein sample fully characterized in terms of dispersity may increase the likelihood of successful crystallisation by improving the predictability of the crystallisation process. To maximize the probability of crystallisation of a novel mouse macrophage protein (rMMP), target molecule was characterized and refined to improve monodispersity. Addition of MgCl 2 at low concentrations resolves the rMMP into a monodisperse solution, and finally successful crystallization of rMMP was achieved. The effect of MgCl2 was studied using gel filtration chromatography and dynamic light scattering.

AB - X-ray crystallography for the determination of three-dimensional structures of protein macromolecules represents an important tool in function assignment of uncharacterized proteins. However, crystallisation is often difficult to achieve. A protein sample fully characterized in terms of dispersity may increase the likelihood of successful crystallisation by improving the predictability of the crystallisation process. To maximize the probability of crystallisation of a novel mouse macrophage protein (rMMP), target molecule was characterized and refined to improve monodispersity. Addition of MgCl 2 at low concentrations resolves the rMMP into a monodisperse solution, and finally successful crystallization of rMMP was achieved. The effect of MgCl2 was studied using gel filtration chromatography and dynamic light scattering.

KW - Crystallisation

KW - Dispersity

KW - Dynamic light scattering

KW - Gel filtration chromatography

UR - http://www.scopus.com/inward/record.url?scp=84896543315&partnerID=8YFLogxK

U2 - 10.1007/s11010-013-1934-x

DO - 10.1007/s11010-013-1934-x

M3 - Article

SN - 0300-8177

VL - 389

SP - 133

EP - 139

JO - MOLECULAR AND CELLULAR BIOCHEMISTRY

JF - MOLECULAR AND CELLULAR BIOCHEMISTRY

IS - 1-2

ER -

Addition of magnesium chloride to enhance mono-dispersity of a coiled-coil recombinant mouse macrophage protein

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this