TY - JOUR
T1 - Advances in the molecular genetic analysis of the flax-flax rust interaction
AU - Ellis, Jeff
AU - Lawrence, Greg
AU - Ayliffe, Michael
AU - Anderson, Peter
AU - Collins, Nick
AU - Finnegan, Jean
AU - Frost, Donna
AU - Luck, Joanne
AU - Pryor, Tony
PY - 1997/9
Y1 - 1997/9
N2 - The L6 and M rust-resistance genes, representing two of the five rust-resistance gene loci in flax (Linum usitatissimum), have been cloned. The molecular data are fully consistent with earlier genetic data: the L locus is a single gene with multiple alleles expressing different rust resistance specificities, and the M locus is complex, containing an array of about 15 similar genes. Thus, while L6 and M resistance genes have 86% nucleotide identity, their locus structure is very different. These genes encode products belonging to the nucleotide binding siteleucine-rich repeat class of disease-resistance proteins. Analysis of alleles from the L locus and chimetic genes is providing evidence suggesting that important specificity determinants occur in the C-terminal half of the proteins, the region containing the leucine-rich repeats. The isolation and characterization of the rust (Melampsora lini) avirulence genes that correspond to the cloned rust-resistance genes is one of the major challenges remaining to the understanding of this system.
AB - The L6 and M rust-resistance genes, representing two of the five rust-resistance gene loci in flax (Linum usitatissimum), have been cloned. The molecular data are fully consistent with earlier genetic data: the L locus is a single gene with multiple alleles expressing different rust resistance specificities, and the M locus is complex, containing an array of about 15 similar genes. Thus, while L6 and M resistance genes have 86% nucleotide identity, their locus structure is very different. These genes encode products belonging to the nucleotide binding siteleucine-rich repeat class of disease-resistance proteins. Analysis of alleles from the L locus and chimetic genes is providing evidence suggesting that important specificity determinants occur in the C-terminal half of the proteins, the region containing the leucine-rich repeats. The isolation and characterization of the rust (Melampsora lini) avirulence genes that correspond to the cloned rust-resistance genes is one of the major challenges remaining to the understanding of this system.
KW - A virulence genes
KW - Complex loci
KW - Gene-for-gene specificity
KW - Plant disease-resistance genes
KW - Resistance gene cloning
UR - http://www.scopus.com/inward/record.url?scp=0030863729&partnerID=8YFLogxK
U2 - 10.1146/annurev.phyto.35.1.271
DO - 10.1146/annurev.phyto.35.1.271
M3 - Review article
AN - SCOPUS:0030863729
VL - 35
SP - 271
EP - 291
JO - Annual Review of Phytopathology
JF - Annual Review of Phytopathology
SN - 0066-4286
ER -