Advax, a Delta Inulin Microparticle, Potentiates In-built Adjuvant Property of Co-administered Vaccines

Masayuki Hayashi; Taiki Aoshi; Yasunari Haseda; Kouji Kobiyama; Edward Wijaya; Noriyuki Nakatsu; Yoshinobu Igarashi; Daron M. Standley; Hiroshi Yamada; Yoshikazu Honda-Okubo; Hiromitsu Hara; Takashi Saito; Toshiyuki Takai; Cevayir Coban; Nikolai Petrovsky; Ken J. Ishii

doi:10.1016/j.ebiom.2016.11.015

Advax, a Delta Inulin Microparticle, Potentiates In-built Adjuvant Property of Co-administered Vaccines

Masayuki Hayashi, Taiki Aoshi, Yasunari Haseda, Kouji Kobiyama, Edward Wijaya, Noriyuki Nakatsu, Yoshinobu Igarashi, Daron M. Standley, Hiroshi Yamada, Yoshikazu Honda-Okubo, Hiromitsu Hara, Takashi Saito, Toshiyuki Takai, Cevayir Coban, Nikolai Petrovsky, Ken J. Ishii

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Abstract

Advax, a delta inulin-derived microparticle, has been developed as an adjuvant for several vaccines. However, its immunological characteristics and potential mechanism of action are yet to be elucidated. Here, we show that Advax behaves as a type-2 adjuvant when combined with influenza split vaccine, a T helper (Th)2-type antigen, but behaves as a type-1 adjuvant when combined with influenza inactivated whole virion (WV), a Th1-type antigen. In addition, an adjuvant effect was not observed when Advax-adjuvanted WV vaccine was used to immunize toll-like receptor (TLR) 7 knockout mice which are unable to respond to RNA contained in WV antigen. Similarly, no adjuvant effect was seen when Advax was combined with endotoxin-free ovalbumin, a neutral Th0-type antigen. An adjuvant effect was also not seen in tumor necrosis factor (TNF)-α knockout mice, and the adjuvant effect required the presences of dendritic cells (DCs) and phagocytic macrophages. Therefore, unlike other adjuvants, Advax potentiates the intrinsic or in-built adjuvant property of co-administered antigens. Hence, Advax is a unique class of adjuvant which can potentiate the intrinsic adjuvant feature of the vaccine antigens through a yet to be determined mechanism.

Original language	English
Pages (from-to)	127-136
Number of pages	10
Journal	EBioMedicine
Volume	15
DOIs	https://doi.org/10.1016/j.ebiom.2016.11.015
Publication status	Published - Feb 2017
Externally published	Yes

Bibliographical note

This is an open access article under the CC BY-NC-ND license(http://creativecommons.org/licenses/by-nc-nd/4.0/).

Keywords

Adjuvant
Inulin
Macrophage
Particle
Vaccine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ebiom.2016.11.015Licence: CC BY-NC-ND

Hayashi_Advax_P2017Final published version, 1.49 MBLicence: CC BY-NC-ND

Cite this

Hayashi, M., Aoshi, T., Haseda, Y., Kobiyama, K., Wijaya, E., Nakatsu, N., Igarashi, Y., Standley, D. M., Yamada, H., Honda-Okubo, Y., Hara, H., Saito, T., Takai, T., Coban, C., Petrovsky, N., & Ishii, K. J. (2017). Advax, a Delta Inulin Microparticle, Potentiates In-built Adjuvant Property of Co-administered Vaccines. EBioMedicine, 15, 127-136. https://doi.org/10.1016/j.ebiom.2016.11.015

@article{da636ff8f5964de2a8e9454dc507b527,

title = "Advax, a Delta Inulin Microparticle, Potentiates In-built Adjuvant Property of Co-administered Vaccines",

abstract = "Advax, a delta inulin-derived microparticle, has been developed as an adjuvant for several vaccines. However, its immunological characteristics and potential mechanism of action are yet to be elucidated. Here, we show that Advax behaves as a type-2 adjuvant when combined with influenza split vaccine, a T helper (Th)2-type antigen, but behaves as a type-1 adjuvant when combined with influenza inactivated whole virion (WV), a Th1-type antigen. In addition, an adjuvant effect was not observed when Advax-adjuvanted WV vaccine was used to immunize toll-like receptor (TLR) 7 knockout mice which are unable to respond to RNA contained in WV antigen. Similarly, no adjuvant effect was seen when Advax was combined with endotoxin-free ovalbumin, a neutral Th0-type antigen. An adjuvant effect was also not seen in tumor necrosis factor (TNF)-α knockout mice, and the adjuvant effect required the presences of dendritic cells (DCs) and phagocytic macrophages. Therefore, unlike other adjuvants, Advax potentiates the intrinsic or in-built adjuvant property of co-administered antigens. Hence, Advax is a unique class of adjuvant which can potentiate the intrinsic adjuvant feature of the vaccine antigens through a yet to be determined mechanism.",

keywords = "Adjuvant, Inulin, Macrophage, Particle, Vaccine",

author = "Masayuki Hayashi and Taiki Aoshi and Yasunari Haseda and Kouji Kobiyama and Edward Wijaya and Noriyuki Nakatsu and Yoshinobu Igarashi and Standley, {Daron M.} and Hiroshi Yamada and Yoshikazu Honda-Okubo and Hiromitsu Hara and Takashi Saito and Toshiyuki Takai and Cevayir Coban and Nikolai Petrovsky and Ishii, {Ken J.}",

note = "This is an open access article under the CC BY-NC-ND license(http://creativecommons.org/licenses/by-nc-nd/4.0/).",

year = "2017",

month = feb,

doi = "10.1016/j.ebiom.2016.11.015",

language = "English",

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Hayashi, M, Aoshi, T, Haseda, Y, Kobiyama, K, Wijaya, E, Nakatsu, N, Igarashi, Y, Standley, DM, Yamada, H, Honda-Okubo, Y, Hara, H, Saito, T, Takai, T, Coban, C, Petrovsky, N & Ishii, KJ 2017, 'Advax, a Delta Inulin Microparticle, Potentiates In-built Adjuvant Property of Co-administered Vaccines', EBioMedicine, vol. 15, pp. 127-136. https://doi.org/10.1016/j.ebiom.2016.11.015

TY - JOUR

T1 - Advax, a Delta Inulin Microparticle, Potentiates In-built Adjuvant Property of Co-administered Vaccines

AU - Hayashi, Masayuki

AU - Aoshi, Taiki

AU - Haseda, Yasunari

AU - Kobiyama, Kouji

AU - Wijaya, Edward

AU - Nakatsu, Noriyuki

AU - Igarashi, Yoshinobu

AU - Standley, Daron M.

AU - Yamada, Hiroshi

AU - Honda-Okubo, Yoshikazu

AU - Hara, Hiromitsu

AU - Saito, Takashi

AU - Takai, Toshiyuki

AU - Coban, Cevayir

AU - Petrovsky, Nikolai

AU - Ishii, Ken J.

N1 - This is an open access article under the CC BY-NC-ND license(http://creativecommons.org/licenses/by-nc-nd/4.0/).

PY - 2017/2

Y1 - 2017/2

N2 - Advax, a delta inulin-derived microparticle, has been developed as an adjuvant for several vaccines. However, its immunological characteristics and potential mechanism of action are yet to be elucidated. Here, we show that Advax behaves as a type-2 adjuvant when combined with influenza split vaccine, a T helper (Th)2-type antigen, but behaves as a type-1 adjuvant when combined with influenza inactivated whole virion (WV), a Th1-type antigen. In addition, an adjuvant effect was not observed when Advax-adjuvanted WV vaccine was used to immunize toll-like receptor (TLR) 7 knockout mice which are unable to respond to RNA contained in WV antigen. Similarly, no adjuvant effect was seen when Advax was combined with endotoxin-free ovalbumin, a neutral Th0-type antigen. An adjuvant effect was also not seen in tumor necrosis factor (TNF)-α knockout mice, and the adjuvant effect required the presences of dendritic cells (DCs) and phagocytic macrophages. Therefore, unlike other adjuvants, Advax potentiates the intrinsic or in-built adjuvant property of co-administered antigens. Hence, Advax is a unique class of adjuvant which can potentiate the intrinsic adjuvant feature of the vaccine antigens through a yet to be determined mechanism.

AB - Advax, a delta inulin-derived microparticle, has been developed as an adjuvant for several vaccines. However, its immunological characteristics and potential mechanism of action are yet to be elucidated. Here, we show that Advax behaves as a type-2 adjuvant when combined with influenza split vaccine, a T helper (Th)2-type antigen, but behaves as a type-1 adjuvant when combined with influenza inactivated whole virion (WV), a Th1-type antigen. In addition, an adjuvant effect was not observed when Advax-adjuvanted WV vaccine was used to immunize toll-like receptor (TLR) 7 knockout mice which are unable to respond to RNA contained in WV antigen. Similarly, no adjuvant effect was seen when Advax was combined with endotoxin-free ovalbumin, a neutral Th0-type antigen. An adjuvant effect was also not seen in tumor necrosis factor (TNF)-α knockout mice, and the adjuvant effect required the presences of dendritic cells (DCs) and phagocytic macrophages. Therefore, unlike other adjuvants, Advax potentiates the intrinsic or in-built adjuvant property of co-administered antigens. Hence, Advax is a unique class of adjuvant which can potentiate the intrinsic adjuvant feature of the vaccine antigens through a yet to be determined mechanism.

KW - Adjuvant

KW - Inulin

KW - Macrophage

KW - Particle

KW - Vaccine

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U2 - 10.1016/j.ebiom.2016.11.015

DO - 10.1016/j.ebiom.2016.11.015

M3 - Article

C2 - 27919753

AN - SCOPUS:85007415529

SN - 2352-3964

VL - 15

SP - 127

EP - 136

JO - EBioMedicine

JF - EBioMedicine

ER -

Advax, a Delta Inulin Microparticle, Potentiates In-built Adjuvant Property of Co-administered Vaccines

Abstract

Bibliographical note

Keywords

UN SDGs

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