TY - JOUR
T1 - Aggregation-Induced Emission Photosensitizer Boosting Algal Growth and Lipid Accumulation
AU - Rakhi, Sharmin
AU - Reza, AHM Mohsinul
AU - Davies, Brynley
AU - Wang, Jianzhong
AU - Tang, Youhong
AU - Qin, Jianguang
PY - 2024/8/19
Y1 - 2024/8/19
N2 - Mass production of microalgae is a research focus owing to their promising aspects for sustainable food, biofunctional compounds, nutraceuticals, and biofuel feedstock. This study uses a novel approach to enhance microalgae-derived biomass and metabolites by using an aggregation-induced emission (AIE) photosensitizer (PS), CN-TPAQ-PF6 ([C32H23N4]+). The unique AIE features of CN-TPAQ-PF6 facilitate nano-aggregation in aquatic media for an effective light spectral shift for photosynthetic augmentation in a green microalga, Chlamydomonas reinhardtii. The high reactive oxygen species (ROS) production capacity and redox-based cellular modulations reveal its potential to upsurge algal growth and lipid biosynthesis and fabricate fatty acid profiles in the metabolic pathways. Algal cells are labeled with other AIE-based nanoprobes, which are suitable as an in vivo visualization toolkit with superior fluorescence. Furthermore, cytotoxicity analysis of CN-TPAQ-PF6 on the HaCat cell line confirms that this AIE PS is biocompatible without adverse impact on living cells. The results demonstrate the property of AIE PS for the first time in enhancing algal growth and lipid accumulation simultaneously.
AB - Mass production of microalgae is a research focus owing to their promising aspects for sustainable food, biofunctional compounds, nutraceuticals, and biofuel feedstock. This study uses a novel approach to enhance microalgae-derived biomass and metabolites by using an aggregation-induced emission (AIE) photosensitizer (PS), CN-TPAQ-PF6 ([C32H23N4]+). The unique AIE features of CN-TPAQ-PF6 facilitate nano-aggregation in aquatic media for an effective light spectral shift for photosynthetic augmentation in a green microalga, Chlamydomonas reinhardtii. The high reactive oxygen species (ROS) production capacity and redox-based cellular modulations reveal its potential to upsurge algal growth and lipid biosynthesis and fabricate fatty acid profiles in the metabolic pathways. Algal cells are labeled with other AIE-based nanoprobes, which are suitable as an in vivo visualization toolkit with superior fluorescence. Furthermore, cytotoxicity analysis of CN-TPAQ-PF6 on the HaCat cell line confirms that this AIE PS is biocompatible without adverse impact on living cells. The results demonstrate the property of AIE PS for the first time in enhancing algal growth and lipid accumulation simultaneously.
KW - aggregation-induced emission
KW - light wavelength
KW - lipid
KW - microalgae
KW - photosensitiser
KW - reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=85201385157&partnerID=8YFLogxK
U2 - 10.1002/smll.202402463
DO - 10.1002/smll.202402463
M3 - Article
AN - SCOPUS:85201385157
SN - 1613-6810
JO - Small
JF - Small
M1 - 2402463
ER -