Aging Differentially Affects Multiple Aspects of Vesicle Fusion Kinetics

Mark Zanin, Madeleine Phillips, Kimberly Mackenzie, Damien Keating

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    How fusion pore formation during exocytosis affects the subsequent release of vesicle contents remains incompletely understood. It is unclear if the amount released per vesicle is dependent upon the nature of the developing fusion pore and whether full fusion and transient kiss and run exocytosis are regulated by similar mechanisms. We hypothesise that if consistent relationships exist between these aspects of exocytosis then they will remain constant across any age. Using amperometry in mouse chromaffin cells we measured catecholamine efflux during single exocytotic events at P0, 1 month and 6 months. At all ages we observed full fusion (amperometric spike only), full fusion preceded by fusion pore flickering (pre-spike foot (PSF) signal followed by a spike) and pure "kiss and run" exocytosis (represented by stand alone foot (SAF) signals). We observe age-associated increases in the size of all 3 modes of fusion but these increases occur at different ages. The release probability of PSF signals or full spikes alone doesn't alter across any age in comparison with an age-dependent increase in the incidence of "kiss and run" type events. However, the most striking changes we observe are age-associated changes in the relationship between vesicle size and the membrane bending energy required for exocytosis. Our data illustrates that vesicle size does not regulate release probability, as has been suggested, that membrane elasticity or flexural rigidity change with age and that the mechanisms controlling full fusion may differ from those controlling "kiss and run" fusion.

    Original languageEnglish
    Article numbere27820
    Pages (from-to)1-10
    Number of pages10
    JournalPLoS One
    Volume6
    Issue number11
    DOIs
    Publication statusPublished - 18 Nov 2011

    Fingerprint Dive into the research topics of 'Aging Differentially Affects Multiple Aspects of Vesicle Fusion Kinetics'. Together they form a unique fingerprint.

  • Cite this