Agrobacterium tumefaciens supports DNA replication of diverse geminivirus types

Luke A. Selth, John W. Randles, M. Ali Rezaian

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We have previously shown that the soil-borne plant pathogen Agrobacterium tumefaciens supports the replication of tomato leaf curl geminivirus (Australian isolate) (TLCV) DNA. However, the reproducibility of this observation with other geminiviruses has been questioned. Here, we show that replicative DNA forms of three other geminiviruses also accumulate at varying levels in Agrobacterium. Geminiviral DNA constructs that lacked the ability to replicate in Agrobacterium were rendered replication-competent by changing their configuration so that two copies of the viral ori were present. Furthermore, we report that low-level replication of TLCV DNA can occur in Escherichia coli containing a dimeric TLCV construct in a high copy number plasmid. These findings were reinforced by expression studies using β-glucuronidase which revealed that all six TLCV promoters are active in Agrobacterium, and two are functional in E. coli.

Original languageEnglish
Pages (from-to)179-182
Number of pages4
JournalFEBS Letters
Volume516
Issue number1-3
DOIs
Publication statusPublished - 10 Apr 2002
Externally publishedYes

Keywords

  • β-Glucuronidase
  • Agrobacterium tumefaciens
  • Begomovirus
  • Escherichia coli
  • Geminivirus
  • Replication

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