Allelic variation contributes to bacterial host specificity

Min Yue, Xiangan Han, Leon De Masi, Chunhong Zhu, Xun Ma, Junjie Zhang, Renwei Wu, Robert Schmieder, Radhey S. Kaushik, George P. Fraser, Shaohua Zhao, Patrick F. McDermott, François Xavier Weill, Jacques G. Mainil, Cesar Arze, W. Florian Fricke, Robert A. Edwards, Dustin Brisson, Nancy R. Zhang, Shelley C. RankinDieter M. Schifferli

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41 Citations (Scopus)

Abstract

Understanding the molecular parameters that regulate cross-species transmission and host adaptation of potential pathogens is crucial to control emerging infectious disease. Although microbial pathotype diversity is conventionally associated with gene gain or loss, the role of pathoadaptive nonsynonymous single-nucleotide polymorphisms (nsSNPs) has not been systematically evaluated. Here, our genome-wide analysis of core genes within Salmonella enterica serovar Typhimurium genomes reveals a high degree of allelic variation in surface-exposed molecules, including adhesins that promote host colonization. Subsequent multinomial logistic regression, MultiPhen and Random Forest analyses of known/suspected adhesins from 580 independent Typhimurium isolates identifies distinct host-specific nsSNP signatures. Moreover, population and functional analyses of host-associated nsSNPs for FimH, the type 1 fimbrial adhesin, highlights the role of key allelic residues in host-specific adherence in vitro. Together, our data provide the first concrete evidence that functional differences between allelic variants of bacterial proteins likely contribute to pathoadaption to diverse hosts.

Original languageEnglish
Article number8754
Number of pages11
JournalNature Communications
Volume6
DOIs
Publication statusPublished - 30 Oct 2015
Externally publishedYes

Bibliographical note

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Keywords

  • microbial pathotype diversity
  • cross-species transmission
  • pathoadaptive nonsynonymous single-nucleotide polymorphisms
  • Allelic variation

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    Yue, M., Han, X., Masi, L. D., Zhu, C., Ma, X., Zhang, J., Wu, R., Schmieder, R., Kaushik, R. S., Fraser, G. P., Zhao, S., McDermott, P. F., Weill, F. X., Mainil, J. G., Arze, C., Fricke, W. F., Edwards, R. A., Brisson, D., Zhang, N. R., ... Schifferli, D. M. (2015). Allelic variation contributes to bacterial host specificity. Nature Communications, 6, [8754]. https://doi.org/10.1038/ncomms9754