Alterations in the glutathione content of mitochondria following short-term forebrain ischemia in rats

Emad Zaidan, Neil R. Sims

    Research output: Contribution to journalArticlepeer-review

    19 Citations (Scopus)


    Total glutathione was measured in mitochondria isolated following 30 min of ischemia and recirculation periods up to 24 h. Mitochondria prepared from the dorsolateral striatum, a region containing many neurons susceptible to short ischemic periods, were compared with those from the paramedian cortex, an ischemia-resistant region. Parallel increases in glutathione content (to approximately 150% of pre-ischemic values) were seen in both regions during the first few hours of recirculation. By 24 h of recirculation, there was a decrease below pre-ischemic values in preparations from the dorsolateral striatum but not the paramedian cortex. The early increases in mitochondrial glutathione were not associated with comparable increases in total tissue glutathione. A shorter (10 min) ischemic period also produced an early increase in mitochondrial glutathione but this was reversed more rapidly to preischemic values. The observed changes indicate post-ischemic modifications of cellular oxidative defences in the two brain regions studied.

    Original languageEnglish
    Pages (from-to)75-78
    Number of pages4
    JournalNeuroscience letters
    Issue number2
    Publication statusPublished - 1 Nov 1996

    Bibliographical note

    Funding Information:
    This work was supported by grants from the National Health and Medical Research Council (Australia), Flinders Research Foundation and Flinders University.

    Copyright 2007 Elsevier B.V., All rights reserved.


    • Cerebral cortex
    • Glutathione
    • Ischemia
    • Mitochondria
    • Oxidative damage
    • Striatum


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